Exam

Question 1

How do you carry out an ABO and RhD type in tubes

Answer 1

Need patients rbcs and plasma

Anti A
Anti B
Anti AB
Anti D1
Anti D2
Rh Control (RC)

A1 cells + plasma
B cells + plasma

Incubate at room temp for 5-10 mins
Centrifuge at R2

Question 2

How do you carry out an ABO and RhD type in gels
(4)

Answer 2

Get a normal gel containing anti A, B, D and control

Add 10ul patients rbcs to each well

Add 50ul reagent rbcs to reverse groups (A1 and B) then add 50ul patient plasma

Centrifuge for 10 minutes

Question 3

How do you carry out a Rh phenotype in tubes

Answer 3

Patient rbcs, reagent rbc controls and anti sera

Anti D
Anti C
Anti c
Anti E
Anti e
Anti K

+ control reagent Biorad cell
- control reagent Biorad cell + anti-sera

Incubate for 5 mins
Centrifuge at P5

Question 4

Rh phenotyp in gels

Answer 4

Find ID card with anti C,c, E, e, K and control present

Add 10 ul (3-5%) patient rbcs to each well

Centrifuge for 10 mins

Question 5

3 panel antigen test
(4)

Answer 5

You need x2 LISS/Coombs Gels (Green lines on gel) -> one for your patient and one for your controls

You need 3 BioRad reagent cells (I, II, III)

Controls = 25ul weak anti-D or 25ul Inert AB to each reagent cell (50ul) on control gel i.e. no patient

Needs to be incubated for 15-20 mins at 37 degrees

Question 6

Antibody identification

Answer 6

You need x2 LISS/COOmbs gel cards (green)

You need x2 Enzyme Panel - NaCl Gels (red)

You need your BioRad Antibody ID panel - untreated (white cap)

You need you BioRad antibody ID panel - papanised (red cap)

Need to add 50ul of each rbc and 25ul patient plasma to each

+ Control needed for untreated and treated panel -> 25ul weak anti-D with 50ul RhD positive cell (pick from panel)

Need to incubate at 37 degrees for 15 minute