Practical 1: Introduction to Blood Groups

Question 1

What two groups do we use to determine blood group

Answer 1

A forward and a reverse group

Question 2

How are the blood cells prepared prior to testing

Answer 2

The test red cells are first diluted in normal saline to approximately 3-5% suspension of red cells

Question 3

Why do we have a reverse group?

Answer 3

To establish the correct ABO type
To double check

Question 4

What are the components of a type A person

Answer 4

Erythrocytes with type A antigen

Plasma with anti-B anibodies

Question 5

What are the components of a type B person

Answer 5

Erythrocytes with type B antigen

Plasma with anti-A antibodies

Question 6

What are the components of a type AB person

Answer 6

Erythrocytes with A and B antigens

Plasma with neither A or B antibodies

Question 7

What are the components of a type O person

Answer 7

Erythrocytes with no antigen

Plasma with A and B antibodies

Question 8

For how long do you need to incubate your tubes?

Answer 8

15 to 30 minutes

Question 9

How much rbcs and anti-sera are needed for the forward groups

Answer 9

1 drop rbc

1 drop anti-sera

Question 10

How much plasma and reagent cells are needed for the reverse groups?

Answer 10

2 drops plasma

1 drop reagent cells

Question 11

What reagent cells are used in the reverse group?

Answer 11

A1 cells

B cells

Question 12

What colour is the anti-A reagent

Answer 12

Blue

Question 13

What colour is the anti-B reagent

Answer 13

Yellow

Question 14

What colour is the anti-AB reagent

Answer 14

Clear

Question 15

What is a positive reaction

Answer 15

Haemolysis

Question 16

How is haemolysis rated

Answer 16

Negative = no haemolysis

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