Testing For Sugars 2.2 Flashcards
What is a non-reducing sugar test used to detect?
Sucrose or other non-reducing sugars
What reagent is used in a non-reducing sugar test?
Benedict’s reagent
What color change indicates a positive result in a non-reducing sugar test?
From blue to green, yellow, orange, or red
What is the principle behind a non-reducing sugar test?
Non-reducing sugars are first hydrolyzed into reducing sugars before being detected
What enzyme is used to hydrolyze non-reducing sugars in a non-reducing sugar test?
Sucrase
What is the purpose of boiling the sample in a non-reducing sugar test?
To denature enzymes that may interfere with the test
What is the purpose of adding hydrochloric acid in a non-reducing sugar test?
To hydrolyze non-reducing sugars into reducing sugars
What is the color of Benedict’s reagent before the test?
Blue
What is the function of sodium carbonate in a non-reducing sugar test?
To neutralize the hydrochloric acid added during the test
What is the color change in Benedict’s reagent without any reducing sugar present?
No color change (remains blue)
What is the color change in Benedict’s reagent with reducing sugar present?
From blue to green, yellow, orange, or red
What type of bond do non-reducing sugars have that prevents them from reducing Benedict’s reagent?
Glycosidic bond
What is the role of boiling water bath in a non-reducing sugar test?
To heat the sample and facilitate the reaction
What is the role of cooling the sample in a non-reducing sugar test?
To stop further reaction and allow for color observation
What is the final color change indicating a positive result in a non-reducing sugar test?
Red
What is the final color change indicating a negative result in a non-reducing sugar test?
No color change or remains blue
What is the term for a substance that can reduce Benedict’s reagent?
Reducing sugar
What is the term for a substance that cannot reduce Benedict’s reagent?
Non-reducing sugar
What is the role of heating the test tube in a non-reducing sugar test?
To promote the reaction of reducing sugars with Benedict’s reagent
What is the importance of using a control in a non-reducing sugar test?
To verify the accuracy of the test results
What is the purpose of centrifuging the sample in a non-reducing sugar test?
To separate solid particles from the solution before testing
What is the role of a spectrophotometer in a non-reducing sugar test?
To quantitatively measure the color change in the sample
What is the role of a colorimeter in a non-reducing sugar test?
To measure the intensity of color change in the sample
What is the significance of a negative result in a non-reducing sugar test?
Indicates the absence of non-reducing sugars in the sample
