Testing For Sugars 2.2

Question 1

What is a non-reducing sugar test used to detect?

Answer 1

Sucrose or other non-reducing sugars

Question 2

What reagent is used in a non-reducing sugar test?

Answer 2

Benedict’s reagent

Question 3

What color change indicates a positive result in a non-reducing sugar test?

Answer 3

From blue to green, yellow, orange, or red

Question 4

What is the principle behind a non-reducing sugar test?

Answer 4

Non-reducing sugars are first hydrolyzed into reducing sugars before being detected

Question 5

What enzyme is used to hydrolyze non-reducing sugars in a non-reducing sugar test?

Answer 5

Sucrase

Question 6

What is the purpose of boiling the sample in a non-reducing sugar test?

Answer 6

To denature enzymes that may interfere with the test

Question 7

What is the purpose of adding hydrochloric acid in a non-reducing sugar test?

Answer 7

To hydrolyze non-reducing sugars into reducing sugars

Question 8

What is the color of Benedict’s reagent before the test?

Answer 8

Blue

Question 9

What is the function of sodium carbonate in a non-reducing sugar test?

Answer 9

To neutralize the hydrochloric acid added during the test

Question 10

What is the color change in Benedict’s reagent without any reducing sugar present?

Answer 10

No color change (remains blue)

Question 11

What is the color change in Benedict’s reagent with reducing sugar present?

Answer 11

From blue to green, yellow, orange, or red

Question 12

What type of bond do non-reducing sugars have that prevents them from reducing Benedict’s reagent?

Answer 12

Glycosidic bond

Question 13

What is the role of boiling water bath in a non-reducing sugar test?

Answer 13

To heat the sample and facilitate the reaction

Question 14

What is the role of cooling the sample in a non-reducing sugar test?

Answer 14

To stop further reaction and allow for color observation

Question 15

What is the final color change indicating a positive result in a non-reducing sugar test?

Answer 15

Red

Question 16

What is the final color change indicating a negative result in a non-reducing sugar test?

Answer 16

No color change or remains blue

Question 17

What is the term for a substance that can reduce Benedict’s reagent?

Answer 17

Reducing sugar

Question 18

What is the term for a substance that cannot reduce Benedict’s reagent?

Answer 18

Non-reducing sugar

Question 19

What is the role of heating the test tube in a non-reducing sugar test?

Answer 19

To promote the reaction of reducing sugars with Benedict’s reagent

Question 20

What is the importance of using a control in a non-reducing sugar test?

Answer 20

To verify the accuracy of the test results

Question 21

What is the purpose of centrifuging the sample in a non-reducing sugar test?

Answer 21

To separate solid particles from the solution before testing

Question 22

What is the role of a spectrophotometer in a non-reducing sugar test?

Answer 22

To quantitatively measure the color change in the sample

Question 23

What is the role of a colorimeter in a non-reducing sugar test?

Answer 23

To measure the intensity of color change in the sample

Question 24

What is the significance of a negative result in a non-reducing sugar test?

Answer 24

Indicates the absence of non-reducing sugars in the sample