Test 1-Quiz 3

Question 0

There should be ____ colony growth on your plate

Answer 0

Uniform

Question 1

______ is an indication of bacterial growth, where broth cultures are cloudy

Answer 1

Turbidity

Question 2

Streak plates should form _____ colonies

Answer 2

Individual

Question 3

Staining is used because the cells are ______

Answer 3

Transparent

Question 4

Staining helps to ____ bt different types of bacteria

Answer 4

Differentiate

Question 5

Simple stain can determine the ___ and ____ of bacteria that can withstand heat fixing

Answer 5

Morphology, arrangement

Question 6

______ helps bacteria adhere to the slide.

Answer 6

Heat-fixing

Question 7

Pros and cons of heat fixing

Answer 7

Pros: kills bacteria making it safe to work with

Cons: causes some distortion of cells.

Question 8

Morphology (shape), 3 types

Answer 8

Cocci-sphere, bacilli-rods, spirilla-spirals

Question 9

Some bacteria grow in specific _____

Answer 9

Arrangements

Question 10

Single, chains, pairs, tetrads, clusters are different

Answer 10

Arrangements

Question 11

Salts with one positively charged ion and one negatively charged ion

Answer 11

Dyes

Question 12

Bacteria is ____ charged, so the cation binds with the bacteria and stains the cell

Answer 12

Negatively

Question 13

____ dye, the bacteria is dyed by the positively charged color, example methylene blue

Answer 13

Basic

Question 14

______ dye, negative charged dyes the background, example Congo red, nigrosin

Answer 14

Acidic

Question 15

The ____ stain determines cell morphology and arrangement without heat-fixing organisms

Answer 15

Negative

Question 16

Negative staining uses an ____ dye, so the ____ will be stained while the ____ appear transparent

Answer 16

Acidic, background

Question 17

The ____ stain is a differential technique that uses both basic and acidic dyes

Answer 17

Capsule

Question 18

Characteristics of the capsule

Answer 18

• Comprised of polysaccharides or polypeptides
• gelatinous
• surrounds the bacterial cell
• tightly packed matrix

Question 20

Purpose of the capsule

Answer 20

• Attachment (for protection)

- increases virulence (protection from phagocytosis)

Question 21

A colony is: (3)

Answer 21

pure culture
derived from one original cell
a clump of identical cells

Question 22

Name a technique to establish a pure culture

Answer 22

streak plate

Question 23

T or F: Pour plates are made using dilute samples of bacteria

Answer 23

True

Question 24

T or F: Pour liquified agar into the petri plate after adding broth culture

Answer 24

True

Question 25

Aseptic

Answer 25

free from microbrial contamination

Question 26

Why is the inoculation loop resteralized between streaks when creating a streak plate?

Answer 26

To kill any bacteria on the loop, this allows you to dilute the bacteria present with subsequent streaks

Question 27

Which of the pipettes would you use to measure our .1mL of culture?

Answer 27

1mL pipette

Question 28

Name two ways that bacilli arrange themselves

Answer 28

singles, chain

Question 29

cocci arrange themselves in

Answer 29

tetrads and clusters

Question 30

Why heat fix bacteria onto microscope slides

Answer 30

help bacteria adhere to the slide

Question 31

Most bacteria is ___ charged, ___ dyes must be used to stain the cell wall

Answer 31

negatively, basic

Question 32

T or F: A negative stain always uses an acidic dye.

Answer 32

False

Question 33

Which stains should not be heat fixed?

Answer 33

Negative and Capsule stain

Question 34

What is the basic dye used in the capsule stain

Answer 34

Manevals stain

Question 35

What is the name of the dye used in the simple stain

Answer 35

Methylene Blue

Question 36

What color is the bacterial cell following the capsule stain

Answer 36

Clear (unstained)