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Question 1

microaerophiles

Answer 1

require a very specific amount of low oxygen

Question 2

device to promote growth of anaerobic and microaerophilic bacteria

Answer 2

anerobe jar

Question 3

indicator strip on anerobe jar contains a dye called

Answer 3

resazurin

Question 4

The strip of resazurin is ___ in presence of oxygen and turns ___ when no oxygen is present

Answer 4

pink, white

Question 5

psychrophiles

Answer 5

organisms that grow at temps less than 20C (ocean, arctic)

Question 6

psychrotrophs

Answer 6

organisms that grow at temps that range from 0-30C

Question 7

mesophiles

Answer 7

organisms that grow at temps ranges from 15c-45c. (human body and in our climate)

Question 8

thermophiles

Answer 8

organisms that grow at temps higher than 40C (hot springs)

Question 9

Extreme thermophiles

Answer 9

organisms that grow at temps above 60C

Question 10

Method for determining cell density of microbial population. Quantitative way of determining cell density.

Answer 10

standard plate count

Question 11

Serial dilution

Answer 11

a series of controlled transfers until the sample density is reduced to 1 millionth or less of its original cell concentration

Question 12

Advantages of serial dilution (3)

Answer 12

-only counts viable cells-can be modified to detect specific organisms-easy to do and cost effective

Question 13

Disadvantages of serial dilution (3)

Answer 13

-takes 24-48 hours for bacteria to grow-can only assume one cell produces one colony-assumes all viable cells grow

Question 14

Initial dilution calculation

Answer 14

D=V1/V2 WHERE D=DILUTION FACTOR, V1=VOLUME OF BROTH BEING DILUTED, AND V2=TOTAL COMBINED VOLUME OF BROTH AND DILUENT

Question 15

SERIAL DILUTION CALCULATION

Answer 15

D2=V1/V2XD1

Question 16

ONLY USE THE SAME PIPETTE WHEN

Answer 16

TRANSFERRING TO THE PETRI PLATE