quiz 9 Flashcards
Phenol Red Broth (PR) contains nutrient broth, carbohydrate, _______, and durham tube
pH indicator (Phenol Red)
________ is used to detect the formation of gas products from fermentation
Durham tube
used to indentify bacteria
enzymes are responsible for biochemical reactins
biochemical testing
Phenol Red has ___ and ___ tubes
lactose, glucose
Hydrogen peroxide is toxic to cells, ____ is produced by aerobic and facultative bacteria to break down the hydrogen peroxide
Catalase
Detects if a bacterium produces a molecule called cytochrome c oxidase
Oxidase test
Test used to identify Neisseria species and Pseudomonas species
Oxidase test
Determines if sodium citrate is used as a sole carbon source by microorganisms
Citrate test
If citrate is used, the medium becomes alkaline and the slant turns ____
blue
Enzymes that remove the amine group from an amino acid are called deaminases
Phenylalanine Deaminase test
Organisms that remove the amine group from phenylalanine do this by producing an enzyme called
phenylalanine
Some microorganisms can hydrolyze (split apart) starch into simple carbohydrates utilizing the enzyme amylase
Starch Hydrolysis test
When starch combines with iodine, a ______ color is formed
purple/black
_____ breaks down starch
amylase
Urea can be hydrolyzed by some microorganisms to carbon dioxide and ammonia by using the enzyme urease
Urea Hydrolysis test
____ indicates NH3 is basic
pink
If SIM medium blackens it indicates the presence of
Hydrogen sulfide
____ is a by-product that is produced when the bacteria hydrolyze tryptophan utilizing the enzyme tryptophanase
Indole
determine motility by ____ inoculation
stab
