Midterm/lab6 Flashcards
Temperature classification
Ps. fluorescens
E.coli
B.stearothermophilis
Temperature classification
Ps. fluorescens-psychrotroph
E.coli-mesophile
B.stearothermophilis-thermophile
Aerotolerance classification:
M.luteus
E.coli
C.sporogenes
Aerotolerance classification:
M.luteus-obligate aerobe
E.coli-facultative anaerobe
C.sporogenes-obligate anaerobe
Standard plate count OCD=
Colonies counted (CFU)/dilution factor (mL)
Disinfectant
Used on inanimate surfaces, strongest concentration
Antiseptic
Used on living tissues, weakest concentration
Can the same chemical be a antiseptic and a disinfectant?
Yes
Longest wavelength 315-400nm
UV-A
Most detrimental wavelength to bacteria
UV-C (100-280nm)
Antimicrobrial susceptibility test
Kirby-Bauer
Natural anti microbial agents produced by microorganisms
Antibiotics
Medium for Kirby Bayer test
Mueller-Hinton agar
Broth culture density based on
McFarland standards
Interpretation of Kirby-Bauer test is to determine if microbe is
Susceptible, resistant, or intermediate to the antibiotic
McFarland standards are used as a way to determine
Cell density based on turbidity