Test 1

Question 0

An example of a biosafety level 4 organism would be

Answer 0

Ebola

Question 1

Organisms that do not typically cause disease in healthy individuals are examples of biosafety level

Answer 1

1

Question 2

Do what upon entering and exiting the laboratory?

Answer 2

Wash hands

Question 3

Disinfect work services using

Answer 3

Wescodyne

Question 4

Produces an image made from light that is transmitted through a specimen

Answer 4

Bright field microscopy

Question 5

What concentrates the light through the specimen by directing it into a sharp cone

Answer 5

Condenser lens

Question 6

Clarity of an image is called

Answer 6

Resolution

Question 7

What is an actual measurement of how far apart two points must be for the microscope to view them as being separate

Answer 7

Resolving power or limit of resolution

Question 8

What is the 10x View piece

Answer 8

Ocular

Question 9

What holds the objectives

Answer 9

Nosepiece

Question 10

Objectives power: scanning

Answer 10

4x

Question 11

Objectives power: low power

Answer 11

10x

Question 12

Objectives power: high dry

Answer 12

40x

Question 13

Objectives power: oil immersion

Answer 13

100x

Question 14

What is the only objective that you can see bacteria at

Answer 14

100x

Question 15

When object is in focus under one objective it is in approximate focus under all objectives

Answer 15

Parfocal

Question 16

Use the coarse knob only at

Answer 16

4x

Question 17

Concentrates light through the specimen by directing it into a sharp cone

Answer 17

Condenser

Question 18

Raises and lowers stage

Answer 18

Coarse focus knob

Question 19

Brings into focus

Answer 19

Fine focus knob

Question 20

On the condenser, adjusts the amount of light going through the specimen

Answer 20

Iris diaphragm

Question 21

Multiply magnification of the ocular times magnification of the objective to get

Answer 21

Total magnification

Question 22

What is the reason for oil

Answer 22

Decreases the amount of light refraction to improve the resolution because oil and glass have the same refractive index

Question 23

Produces an image made of light that is reflected off of the specimen

Answer 23

Dark field microscopy

Question 24

Produces an image of various levels of darks against a bright background

Answer 24

Phase contrast

Question 25

Produces an image when a fluorescent dye is illuminated with a UV light

Answer 25

Florescence

Question 26

Visible current caused by water molecules colliding with the microorganisms

Answer 26

Brownian movement

Question 27

Free from microbial contamination

Answer 27

Aseptic

Question 28

Purpose of aseptic techniques- to transfer bacteria without

Answer 28

Contaminating the environment, yourself, bacteria culture, or the media

Question 29

In a agar slant, start inoculation at the bottom and

Answer 29

Move upward in a zig zag pattern

Question 30

3 techniques for plate media

Answer 30

Spot, line, lawn

Question 31

A clump of identical cells derived from one original cell

Answer 31

Colony

Question 32

Contain only one species of bacteria

Answer 32

Pure culture

Question 34

The purpose of the pour plate method is

Answer 34

Achieve individual colonies of a dilute sample

Question 35

The ocular of your microscope has a magnification power of

Answer 35

10x

Question 36

Name one organismal trait you were able to determine with the wet-mount preparation

Answer 36

determine motility

Question 37

T or F: Normal skin flora is deeply embedded in our skin and is difficult to remove by handwashing alone

Answer 37

True

Question 38

Which objective lens is the most useful for studying bacterial cells?

Answer 38

100x

Question 39

Which 2 types of microscopy was used to observe the wet mount?

Answer 39

Bright and Dark field

Question 40

REVIEW parts of microscope from quiz 1

Answer 40

arm, iris, etc