TCA Cycle

Question 1

Prokaryotes

Answer 1

Cytoplasm

Question 2

Eukaryotes

Answer 2

Mitochondria

Question 3

Pyruvate to Acetyl-CoA and CO2

Answer 3

pyruvate dehydrogenase complex (E1 + E2 + E3)

Question 4

Step 1

Answer 4

Acetyl-CoA and oxaloacetate condense to citryl-CoA and then hydrolysis to create citrate (release free CoA)

Question 5

Acetyl-CoA to Citrate

Answer 5

Citrate synthase

Question 6

Step 2

Answer 6

Citrate dehydrated to cis-aconitate than rehydrated into isocitrate.

Question 7

Citrate to isocitrate

Answer 7

Aconitase

Question 8

Step 3

Answer 8

1. Dehydrogenation of isocitrate oxalosuccinate
2. Decarboxylation of oxalosuccinate to α-ketoglutarate

Question 9

Isocitrate to α-ketoglutarate

Answer 9

isocitrate dehydrogenase

Question 10

Step 4

Answer 10

α-ketoglutarate oxidatively decarboxylated to succinyl-CoA & CO2

Question 11

α-ketoglutarate to succinyl-CoA

Answer 11

α-ketoglutarate dehydrogenase

Question 12

Step 5

Answer 12

Succinyl-CoA cleaved to form succinate & free CoA

Question 13

Succinyl-CoA to succinate

Answer 13

succinyl-CoA synthase

Question 14

Step 6

Answer 14

Succinate to fumerate

Question 15

Succinate to fumarate

Answer 15

succinate dehydrogenase

Question 16

Step 7

Answer 16

Fumarate hydrated to form L-malate

Question 17

Fumarate to L-Malate

Answer 17

fumarate hydratase

Question 18

Step 8

Answer 18

L-malate dehydrogenated to oxaloacetate

Question 19

L-malate dehydrogenated to oxaloacetate

Answer 19

L-malate dehydrogenase

Question 20

Final Products Each Turn

Answer 20

*3 NADH,
*1 FADH2,
*1 GTP (or ATP)
*2 CO2

Question 21

Reversible Reactions

Answer 21

1,2,5,6,7,8

Question 22

Irreversible Reactions

Answer 22

3,4

Question 23

Produce NADH

Answer 23

3,4,8

Question 24

Produce FADH

Answer 24

6

Question 25

Produce GTP/ATP

Answer 25

5