TCA, B-oxidation, oxidative phosphorylation Flashcards

1
Q

TCA cycle rxn 1 type

A

C-C bond formation by claisen condensation

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2
Q

TCA cycle rxn 1 enzyme

A

citrate synthase

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3
Q

TCA cycle rxn 1

A

acetyl-coA + oxaloacetate = citrate

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4
Q

TCA cycle rxn 2 type

A

isomerization

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5
Q

TCA cycle rxn 2 enzyme

A

aconitase

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6
Q

TCA cycle rxn 2

A

citrate to isocitrate
(cis-aconitase intermediate)

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7
Q

TCA cycle rxn 2 reason

A

citrate is a poor substrate for oxidation
isocitrate has secondary -OH which can be oxidized

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8
Q

citrate chirality

A

prochiral: aconitase removes pro-R H of pro-R arm

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9
Q

TCA cycle rxn 3 type

A

oxidative decarboxylation

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10
Q

TCA cycle rxn 3 enzyme

A

isocitrate dehydrogenase
- link to e- transport chain

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11
Q

TCA cycle rxn 3

A

isocitrate to a-ketoglutarate

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12
Q

TCA cycle rxn 3 intermediate

A

oxalosuccinate

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13
Q

TCA cycle rxn 4 type

A

oxidative decarboxylation

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14
Q

TCA cycle rxn 4 enzyme

A

a-ketoglutarate dehydrogenase
- complex

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15
Q

TCA cycle rxn 4

A

a-ketoglutarate to succinyl-coA

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16
Q

TCA cycle rxn 5 type

A

substrate-level phosphorylation

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17
Q

TCA cycle rxn 5 enzyme

A

succinyl-coA synthetase

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18
Q

TCA cycle rxn 5

A

succinyl-coA to succinate
- synthesis is driven by hydrolysis of coA ester

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19
Q

TCA cycle rxn 5 product type

A

nucleoside triphosphate

20
Q

TCA cycle rxn 6 type

A

oxidation involving FAD

21
Q

TCA cycle rxn 6 enzyme

A

succinate dehydrogenase

22
Q

TCA cycle rxn 6

A

succinate to fumarate

23
Q

TCA cycle rxn 6 FAD

A

FAD to FADH2
part of e- transport chain

24
Q

TCA cycle rxn 7 type

A

hydration across double bond

25
TCA cycle rxn 7 enzyme
fumarase
26
TCA cycle rxn 7
fumarate to L-malate - carbanion transition state
27
TCA cycle rxn 8 type
carbon oxidation
28
TCA cycle rxn 8 enzyme
malate dehydrogenase
29
TCA cycle rxn 8
L-malate to oxaloacetate
30
TCA cycle rxn 8: NAD+
NAD+ to NADH + H+
31
B-oxidation step 1 type
oxidation of Ca-Cb bond
32
B-oxidation step 1 enzyme
acyl-coA dehydrogenase
33
B-oxidation step 1 hydride removal
by FAD
34
B-oxidation step 1 rxn
Palmitoyl-coA to trans-delta-Enoyl-coA
35
B-oxidation step 2 type
water added across double bond
36
B-oxidation step 2 enzyme
enoyl-coA hydratase
37
B-oxidation step 2 rxn
trans-delta-enoyl-coA to L-B-hydroxyacyl-coA
38
B-oxidation step 3 type
oxidation of B-hydroxyl group
39
B-oxidation step 3 enzyme
hydroxyacyl-coA dehydrogenase
40
B-oxidation step 3 cofactor
NAD+
41
B-oxidation step 3 rxn
L-B-hydroxyacyl-coA to B-ketoacyl-coA
42
B-oxidation step 4 type
thiolase/reverse claisen condensation
43
B-oxidation step 4 enzyme
thiolase
44
B-oxidation step 4 rxn
B-ketoacyl-coA to acyl coA and acetyl-coA
45
hormone regulation: epinephrine
need energy
46
hormone regulation: glucagon
need glucose
47
ketone body formation
lack of oxaloacetate acetyl-coA converted into ketone bodies