taxonomy Flashcards
what is nomenclature
the naming of organisms according to established rules and guidlines. latinized names are most commonly used.
what is classification
grouping together organisms that have similarmorphologic, physiologic, and genetic traits
identification of an organism meaning
the process where an organism’s key features; phenotypic and genotypic features are described and portrayed precisely. Identification can be done down to the species, genus or family level
role of taxonomy in diagnostic microbiology
- establishes and maintains records of key characteristics, traits like symptoms, virulence, strains etc of clinically relevant micro organisms
- facilitates in communication between different fields and countries by assigning universally understood names.
this sharing of documented knowledge can lead to
-establishing association between organism and syndrome/disease/condition
- understand how to manage and the outcome of the disease
- recognize new and emerging organisms, and strains and overall changes in types of infections and diseases
-develop new techniques for identification, detection, prevention and medication
role of taxonomy in diagnostic microbiology
- establishes and maintains records of key characteristics, traits like symptoms, virulence, strains etc of clinically relevant micro organisms
- facilitates in communication between different fields and countries by assigning universally understood names.
this sharing of documented knowledge can lead to
-establishing association between organism and syndrome/disease/condition
- understand how to manage and the outcome of the disease
- recognize new and emerging organisms, and strains and overall changes in types of infections and diseases
-develop new techniques for identification, detection, prevention and medication
phenotypic criteria for identification
- macroscopic morphology
microscopic morphology
staining characteristics
antigenic properties
resistance profiles
nutritional and environmental requirements
3 principles for why bacterial cultivation is carried out
- obtain sufficient growth of cliniclaly relevant bacteria for identification and characterization
- determine which of the bacteria in the specimen is most likely causing infection, contaminants and colonizers
- grow and isolate bacteria present in specimen
types of media:
seperated by consistency: solid, semi solid and liquid
seperated by chemical composition: basal, enriched, selective, differential, transport, storage
uses of different types of media
basal -enough for non fastiduous bacteria
enriches- basal w/ more nutrients
selective: eliminates variety of microorganisms that risde on sampling site, (irrelevant , large nos) like commensal bacteria. Has inhibitory agent that goes against growth of irrelevant bacteria.
differential: differentiates closely related organisms by colour; using dyes and chemicals, organism will produce characteristic hcanges in colour.
transport: allows for viability but not replication in case of transport is necessary
storage: retain their properties (usually liquid)