disinfection and sterilization Flashcards

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1
Q

disinfectant slection criteria

A
  • fast acting in the presence of organic substances
  • effective against all types of infectious agents without destroying tissue
  • easily penetrate material to be disinfecting without damaging or discolouring it
  • easy to prepare, stable when exposed to light, heat and other factors
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2
Q

methods of sterilization

A

dry heat- hot air oven- used for glassware, instruments, paper wrapped articles, oils, waxes and powders- 160 for 2 hrs or 170 for 1 hr

moist heat: autoclave and pressure cooker. used for culture media, water containing materials and lab waste.

incineration: burns and physcially destroys living organsism, inoculating loops and lab waste

filtration: used in media prep , filtering delicate supplements that are inactivated by autoclaving(carbs, antibiotics)

UV- used additionally with other methods, sterilize workspaces and tools, germicidal wavelengths 185nm-265nm.

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3
Q

autoclave temp and time

A

121 celcius and 15 mins

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4
Q

2 types of autoclaves

A

porous load autoclaves - modern, fast, reliable efficient, air removed by partial vacuum, admitting steam and reppeating, short cycles

downward displacment autocalve- air removed by dilution in turbulent steam flow, long cycles, inefficient

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