Systemic Lupus Erythematosus Flashcards
SLE prototype of human autoimmune diseases is associated with
HLA DR3
Chronic Inflammatory autoimmune diseases characterized by presence of (SLE) ____
Antinuclear Antibody (ANA)
SLE usual symptoms are
Joint (symmetric arthritis)
Butterfly rashes
Most common visceral organ involved in SLE
Kidney
Major cause of death
Nephritis
Most dangerous lesion
Diffused Renal Proliferation Glomerulonephritis
Laboratory Diagnosis for SLE (3)
- Demonstration of LE Cells
- Fluorescence Anti Nuclear Antibody (FANA)
- Indirect Immunofluorescence
Detection of neutrophil that engulfed the antibody-coated nucleus of another neutrophil
Demonstration of LE cell
Screening test for ANA. It is sensitive but NOT SPECIFIC.
Fluorescence ANA test
Benchmark of autoantibodies present in SLE
Indirect Immunofluorescence
Substrate used in Indirect Immunofluorescence to detect anti-dsDNA
Crithidia lucillae
Crithidia lucillae consist of __________ that aids in the diagnosis of SLE
circular dsDNA
Autoantibody present in Peripheral or Homogenous pattern associated in SLE
Anti-dsDNA
Autoantibody present in Homogenous pattern associated in Drug-induced SLE
Anti-histone
Anti-DNP
Autoantibody present in Coarse speckled pattern associated in
a. SLE
b. Mixed Connective Tissue
Anti-Smith
Anti-RNP
Autoantibody present in Fine speckled pattern associated in Sjoren’s Syndrome and SLE
Anti-SS A/Ro
Anti-SS B/La
Autoantibody present in Prominent Nucleoli Staining pattern associated in Systemic sclerosis
Anti-nucleolar
Autoantibody present in Atypical pattern associated in Systemic sclerosis & scleroderma
Anti-scl-70
Autoantibody present in Fine cytoplasmic SPECKLING associated with Polymyositis
Anti-Jo-1
Autoantibody present in Discrete speckled associated with CREST Syndrome
Anti-centromere
PATTERN FLUORESCENCE:
Uniform staining of nucleus;
Presence of Anti-dsDNA, Anti-Histone, Anti-DNP
Homogenous/Diffuse Pattern
PATTERN FLUORESCENCE:
Intense staining on the outline of the nucleus
Presence of Anti-dsDNA
Rim/Outline Pattern
PATTERN FLUORESCENCE:
Fine/Coarse speckled staining of nucleus;
Presence of Anti-ENA
Speckled Pattern
PATTERN FLUORESCENCE:
Prominent staining of nucleoli
Presence of Anti-RNA, Anti-RNP
Nucleolar Pattern
PATTERN FLUORESCENCE:
Numerous Discrete Speckles
Presence of anti-centromere
Centromere Pattern
CREST Syndrome stands for
Calcinosis
Reynauds Phenomenon
Esophangeal dysmotility
Sclerodactyly
Telangiectasia
Most specific for SLE
Anti-dsDNA antibodies
In SLE, serum complement is
INCREASED OR DECREASED
Decreased (Consumed by Immune-complexes)
