HLA Antibody Detection

Question 1

Majority of HLA Antibodies belongs to the

Answer 1

IgG

Question 2

Uknown serum is added to a

Answer 2

Panel of antigen with known HLA phenotype

Question 3

A panel of at least ____ is required for Initial screening

Answer 3

30 cells

Question 4

A panel of at least ____ is required for Antibody identification

Answer 4

60 cells

Question 5

Microlyphocytotoxicity Test:

Alloantisera reagent uses

Answer 5

CDC
Standard Complement-dependent microlymphocytotoxicity Test

Question 6

Microlyphocytotoxicity Test:

Screening for Recipient serum

Answer 6

Amos-modified Techniques
Extended Incubation
AHG Test

Question 7

Process of washing cell after initial serum incubation for removing anti-complementary activity of serum

Answer 7

Amos-modified

Question 8

AHG added in the CDC modification will bind to the _____ chain of IgG, promoting ______

Answer 8

Light Chain
Complement Activation

Question 9

AHG added in the COOMBS TEST will bind to the _____ chain of IgG, promoting ______

Answer 9

Heavy Chain
Agglutination

Question 10

Can distinguish IgM and IgG isotype
Distinguish non-HLA and HLA antibodies
More sensitive
Cannot be used solely for clinical-decision making

Answer 10

Newer Detection method for HLA Antibody Testing

Question 11

Why does Final crossmatching for specific donor is still needed even if newer method for HLA antibody is used

Answer 11

Not all HLA antigen are present in pool/panel

Question 12

Uses Purified HLA antigens instead of Lymphocytes with 2 types of Assay:

1. Screening Assay
2. Specificity Determination Assay

Answer 12

ELISA
Enzyme Linked Immunoabsorbent Crossmatch Assay

Question 13

ELISA
uses purified HLA antigen to detect anti-HLA antibodies

Answer 13

Screening Assay

Question 14

ELISA
uses purified HLA antigen to detect antibody specificity

Answer 14

Specificity Determination Assay

Question 15

Most sensitive technique in HLA antibody screening

Answer 15

Flow Cytometric Antibody Screen

Question 16

Flow cytometric Antibody Screen uses

Answer 16

T and B Lymphocytes
Purified HLA antigen

Question 17

Flow cytometric Antibody Screen is most effective since it does not need to activate Complement, it directly detects antibody binding

T/F

Answer 17

True

Question 18

HLA antibody screen by which the patient sera is tested against recombinant HLA antigen panel each is bounded with MICROBEADS and labeled with FLUOROPHORE

Answer 18

Multiplex Immunoassay (LUMINEX)

Question 19

Positive Reaction for Multiplex Immunoassay

Answer 19

Mean Fluorescence Intensity

Question 20

Percentage of cells in the panel that the serum was capable of binding

Answer 20

Panel Reactive Antibody

Question 21

PERCENT PRA is represented by

Answer 21

Percentage of Pooled beads (react + with serum)

Question 22

Proportion of population to which patient will react via PREEXITING ANTIBODIES

Answer 22

Panel Reactive Antibody

Question 23

People with High PRA do not need to wait longer for Organ transplant

T/F

Answer 23

False

Individual w/ high PRA tends to wait longer for an Organ since they have wide percentage antibodies that can react against large number of population