Systemic Bacteriology 1 Flashcards

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1
Q

In what ways are microorganisms classified in terms of appearance?

A

Shape
Size
Arrangement
Cell wall (gram + or -)

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2
Q

In what ways are microorganisms classified in terms of growth requirements?

A

Aerobic/anaerobic
Requirement for blood products
Sensitivity to inhibitory proteins

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3
Q

In what ways are microorganisms classified in terms of enzyme or metabolic tests?

A

Coagulase test, catalase test
Haemolysis
Biochemical profiling

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4
Q

In what ways are microorganisms classified in terms of molecular tests?

A

Immunological tests
DNA sequencing
Protein profiling

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5
Q

How are microorganisms identified?

A

Microscopy

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6
Q

What are the most common shapes of bacteria?

A

Cocci - spheres
Bacilli - rods
Spiral shaped

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7
Q

In what way do cocci divide?

A

Divide in one plane to produce two cocci

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8
Q

What are the two structures that are produced when cocci divide and how many planes does each divide in?

A

Chains - one plane

Clumps - three planes

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9
Q

What are bacteria that look like curved rods called?

A

Vibrio

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10
Q

Are vibrio gram positive or negative?

A

Negative

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11
Q

What are the two types of spiral shaped bacteria and what are their names?

A

Rigid - spirillum

Flexible - spirochaete

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12
Q

What is a gram stain?

A

A simple method that distinguishes the two major classes of bacteria (gram + and -) according to cell wall structure

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13
Q

What are the limitations of gram stains?

A

Not all organisms stain well with gram stain

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14
Q

What are the examples of organisms that don’t stain well with gram stains?

A
Mycobacterium tuberculosis
Treponema pallidum (causes syphilis)
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15
Q

What are obligate aerobes?

A

Bacteria that require oxygen

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16
Q

What are obligate anaerobes?

A

Bacteria that are killed by oxygen

17
Q

What are facultative anaerobes?

A

Bacteria that tolerate oxygen

18
Q

The presence of which specific substances permit the growth of one organism over another?

A

Mannitol Salt agar (MSS) allows preferential isolation of staphylococci
Salmonella-Shigella (SS) bile salts inhibit coliforms

19
Q

What are examples of differential media?

A

MacConkay Agar

Eosin and Methylene Blue

20
Q

What is a serological test?

A

A test carried out on a sample of blood seum

21
Q

What is agglutination?

A

The clumping of particles that occurs when an antigen is mixed with its corresponding antibody

22
Q

What may DNA sequencing allow?

A

Precise identification

23
Q

What does MLST stand for?

A

Multilocus Sequence Typing

24
Q

What is MLST?

A

A technique for the typing of multiple loci

25
Q

Why is MALDI-TOF used?

A

Powerful - 99% correct
Rapid
Precise
Cost-effective

26
Q

What is the process of MALDI-TOF?

A

Generates a series of ions from a sample dependent on its constituents
Separates the ions according to mass and charge
Detects the spectrum of proteins released from a sample
Results in a characteristic signature

27
Q

What are four examples of specific microbiology tests?

A

Blood culture
Urine culture
Faeces culture
Swab of pus

28
Q

What are examples of DNA sequencing?

A

qPCR

16sRNA