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HEMATOLOGY > Sysmex/CBC Review > Flashcards

Sysmex/CBC Review Flashcards

1
Q

The electronic impedance cell counter depends on:

A

The cell being less conductive than the isotonic diluent

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2
Q

What best describes the cell counting principle of Sysmex automated blood analyzers?

A

Direct current and hydrodynamic focused flow impedance

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3
Q

What is the type of measurement utilized by the Sysmex system for Hemoglobin?

A

measured photometrically & directly

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4
Q

What information does forward scatter provide about a cell?

A

Measures cell size

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5
Q

How is the MCH calculated?

A

HGB/RBC

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6
Q

How is the MCV calculated?

A

HCT X 1000/RBC

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7
Q

How is the MCHC calculated?

A

HGB/HCT

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8
Q

How is the HCT calculated?

A

MCV X RBC COUNT/1000

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9
Q

RULE OF 3, HCT FORMULA

A

HGB X 3/1000 = HCT +/- 0.03 L/L

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10
Q

RULE OF 3, HGB FORMULA

A

RBC X 30 = HGB +/- 15G/L

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11
Q

Why do laboratories subscribe to External Proficiency Testing?

A

It is further check of result accuracy

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12
Q

What best describes the method used to determine the total WBC counts on the Sysmex analyzers?

A

Fluorescence flow cytometry

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13
Q

What method is used to measure Hemoglobin on an automated cell analyzer?

A

Photometric method

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14
Q

What is the type of measurement utilized by the Sysmex system for WBC?

A

Measured directly using impedance technology

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15
Q

Drabkin’s reagent for the measurement of hemoglobin by the cyanmethemoglobin uses the following reagents.

A

Potassium ferricyanide and potassium cyanide

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16
Q

What best explains/demonstrates a QC “shift”?

A

A sudden change in QC values that are consecutive either above or below the mean

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17
Q

What information does Side scatter provide about a cell?

A

Cytoplasmic granularity and nuclear configuration

18
Q

What is Low Voltage DC used to measure?

A

Total cell volume

19
Q

What is the type of measurement utilized by the Sysmex system for Hematocrit?

A

Measured directly by rbc cumulative pulse height detection

20
Q

What is the type of measurement utilized by the Sysmex system for RBC?

A

measured directly using impedance – DC detection

21
Q

Automated differential analysis can be based on?

A

Granularity of WBC cytoplasm, conductivity, light scattering

22
Q

What is the type of measurement utilized by the Sysmex system for MCH?

A

Calculated using directly measured or derived parameters

23
Q

What method is used to measure RBC/ size and number on the Sysmex Cell analyzer?

A

Direct Current/ Hydrodynamic focusing

24
Q

What best describes the method used to detect the presence of immature myeloid cells on the Sysmex cell analyzer?

A

Radio frequency / Direct current

25
Q

Floating discriminators and/or thresholds (electronically set size limits) are used to

A

distinguish between RBC’s and platelets

26
Q

The cyanmethemoglobin method of hemoglobin determination measures all forms of hemoglobin EXCEPT:

A

Sulfhemoglobin

27
Q

What is laminar flow?

A

movement of two liquids past one another without mixing

28
Q

What is the basis for the impedance principle of cell counting and sizing?

A

Cells create a resistance, when passed through an electrical current that is directly proportional to the volume or size of that cell

29
Q

What action needs to be taken with a critical hemoglobin value?

A

Check for a clot and repeat the test

30
Q

What action is recommended when platelet aggregates or platelet satellitosis are found on the peripheral blood smear?

A

Recollect specimen 4.5 ml whole blood in a 3.2% Sodium Citrate and multiple platelet results by 1.1

31
Q

Which CBC parameters best indicate a possible iron deficiency?

A

↓HGB & ↓MCV & ↑RDW

32
Q

How are CBC results affected by cold agglutinins?

A

Falsely decreasing the HCT

33
Q

Under what conditions would an RBC histogram from an automated cell analyzer shift to the right?

A

Megaloblastic anemia

34
Q

Which RBC parameters indicate normochromic RBCs?

A

MCHC, MCH

35
Q

What parameter best indicates the presence of red cell agglutination?

A

MCHC

36
Q

Which of the following would result in falsely high hemoglobin?

A

A greatly elevated WBC count

37
Q

When examining the peripheral blood smear and Complete Blood count, which parameter should be examined after the hemoglobin and Hematocrit?

A

MCV

38
Q

Under what conditions would an RBC histogram from an automated cell analyzer shift to the left?

A

Chronic blood loss

39
Q

Which CBC parameters best indicate a possible thalassemia?

A

↑RBC,↓MCV, & HGB = N

40
Q

When reviewing a CBC result with a normal MCV, which CBC parameter best alerts the operator that the results may not be accurate?

A

MCHC

HEMATOLOGY (8 decks)

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