Synthetic dyes Flashcards

1
Q

Synthetic dyes are sometimes known as

A

Coal Tar Dyes

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2
Q

Synthetic dyes are derived from the hydro-carbon benzene and are collectively known as

A

Aniline Dyes

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3
Q

substances with definite atomic groupings and are
capable of producing visible colors

A

Chromophores

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4
Q

Simple benzene compounds which contain
such substances are known as

A

chromogens

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5
Q

an auxiliary radical or substance which imparts to the compound the property of electrolytic dissociation, thereby altering the shade of the dye, enabling it to form salts with another compound, and ultimately retaining its color.

A

auxochrome

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6
Q

A dye, therefore, should consist of a

A

chromophore and an auxochrome

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7
Q

where the active coloring substance is found in the acid
component, and the inactive base

A

Acid Dye

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8
Q

usually the sodium salt of a sulfonate of rosaniline

A

acid fuchsin

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9
Q

Acid dye that has the ability to form salt with an alkali.

A

picric acid

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10
Q

only substance so far that can fix, differentiate and stain tissue all by itself

A

Picric acid

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11
Q

It may be employed as a
counterstain to basic cytoplasmic stains; (2)

A
  1. acid fuchsin in Van Gieson’s connective tissue staining
  2. crystal violet for the microscopic study of
    fungi
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12
Q

used as a fixative, as a decalcifying agent, or as a tissue softener

A

Picric acid

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13
Q

type of fixed tissues that are usually take in acidic dyes more readily (3)

A
  1. Trichloracetic acid
  2. picric acid
  3. chromium-fixed
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14
Q

Basic cell structures (collagen, eosinophilic granules
of leukocytes, etc.) have an affinity for the ; and regarded as

A

Acid dye ions ; ACIDOPHILIC

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15
Q

the active coloring substance is found in a basic
component that combines with the acid radical (sulfuric, acetic or hydrochloric acid)

A

Basic Dyes

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16
Q

example of a basic nuclear stain is

A

methylene blue

17
Q

Basic dye that is used both as an indicator and dye

A

methylene blue

18
Q

methylene blue is very widely used in

A

microbiology for bacterial staining.

19
Q

Tissues fixed with __________ and _____usually favor staining with basic dyes.

A

mercuric chloride and formaldehyde

20
Q

Acidic cell structures (chromatin, mucus, cartilage matrix etc.) have an affinity for ________ and are therefore regarded as _____.

A

basic dye ions ; basophilic

21
Q

formed by combining aqueous solutions of acid and
basic dyes, capable of staining cytoplasm and nucleus simultaneously and differentially

A

Neutral Dyes

22
Q

T/F.
Neutral Dyes insoluble or barely soluble in water, but they are usually soluble in alcohol.

A

True

23
Q

Tissues that fixed with ______ readily take in both basic and acidic dye

A

Ethyl alcohol or acetic acid-fixed

24
Q

Examples of neutral dyes are (3)

A
  1. Romanowsky dyes
  2. Giemsa’s stain
  3. Irishman’s stain
25
Q

neutral dye used in hematology

A

Romanowsky dye

26
Q

neutral dye used for leukocyte differentiation (2)

A
  1. Giemsa’s stain
  2. Irishman’s stain