Hematoxylin - Iron Hematoxylin Flashcards
Iron hematoxylin compounds are used only for (2)
- differential
- regressive staining
using _______ as a differentiating agent.
acid-alcohol
Iron hematoxylin:
Two main iron hematoxylin solutions are employed for routine work in the laboratory:
- Weigert’s Solution
- Heidenhain’s Solution
Uses ferric ammonium chloride as mordant
Weigert’s solution
Uses ferric ammonium SULFATE as mordant
Heidenhain’s solution
The dye lake obtained when ferric salts are used as mordants is an
intense blue-black one
Type of Iron Hematoxylin stain used for Mitochondria
Regaud’s hematoxylin
Regaud’s hematoxylin is used for tissues fixed in (2)
- Potassium dichromate
- Formalin
Disadvantage of Regaud’s hematoxylin
Result are not uniform (over stained and under stained)
__________ is the standard iron hematoxylin stain used in the laboratory
Weigert’s solution
Weigert’s solution is used for the demonstration of (2)
Muscle fibers
Connective tissues
Iron hematoxylin stain that is known as a popular cytological stain
Heidenhain’s hematoxylin
Heidenhain’s hematoxylin is used for (2)
- Study of mitosis
- chromatin material
T/F.
Heidenhain’s hematoxylin is used for regressive staining of thin sections
True