Phosphotongstic Acid Hematoxylin (PTAH)

Question 1

Phosphotongstic Acid Hematoxylin (PTAH) uses what mordant

Answer 1

1% aqueous phosphotungstic acid

Question 2

Natural ripening of the tungsten hematoxylin solution is achieved with _______

Answer 2

light and air

Question 3

T/F.
In PTAH when hematin is used instead of hematoxylin, oxidation process is not necessary and the staining solution can be used immediately, but its staining activity is comparatively short-lived.

Answer 3

True

Question 4

In PTAH what are the tissues that stained blue (4)

Answer 4

Nuclei
Fibrin
Muscle striations
Myofibril

Question 5

In PTAH what are the tissues that stained orange-red, brownish red, deep brick-red (3)

Answer 5

Collagen
Bone
Cartilage

Question 6

In PTAH, this substance is added to remove red component of stain

Answer 6

95% alchol

Question 7

Phosphotungstic acid hematoxylin stain usually demonstrates structures in (3))

Answer 7

Paraffin
Celloidin
Frozen sections

Question 8

PTAH staining time

Answer 8

12-24 hrs