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MTAP 2 - STAINING ; FINALS > Other Stain > Flashcards

Other Stain Flashcards

1
Q

mixture of picric acid and acid fuchsin for demonstration of connective tissues.

A

Acid Fuchsin-Picric Acid (Van Gieson’s Stain)

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2
Q

used to stain collagen, smooth muscle, or
mitochondria

A

Acid Fuchsin (Masson Stain)

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3
Q

In Van Gieson’s picro-fuchsin, acid fuchsin imparts its _______ to collagen fibers

A

red color

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4
Q

basic acridine fluorochrome which permits
discrimination between dead and living cells

A

ACRIDINE ORANGE

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5
Q

Acridine orange imparts _____ fluorescent in DNA

A

green

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6
Q

Acridine orange imparts _____ fluorescent in RNA

A

Red

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7
Q

used to demonstrate deposits of calcium salts and possible sites of phosphatase activities

A

ACRIDINE RED 3B

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8
Q

a complex, water-soluble phthalocyanin dye, similar to chlorophyll

A

ALCIAN BLUE

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9
Q

ALCIAN BLUE stains (3)

A
  1. acid mucopolysaccharides
  2. connective tissue
  3. epithelial mucin
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10
Q

Alcian blue is often combined with ______

A

PAS

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11
Q

Alcian blue with PAS stains acidic mucins ______

A

blue

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12
Q

Alcian blue with PAS stains neutral mucin _______

A

Red

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13
Q

forms an orange-red lake with calcium at a pH of 4.2

A

ALIZARIN RED S

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14
Q

ALIZARIN RED S works best with small amounts of calcium such as ___________

A

Michaelis-Gutman
bodies

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15
Q

cytoplasmic stain used for counterstaining of epithelial
sections

A

ANILINE BLUE

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16
Q

Nuclei are deep red; cytoplasm is a pale red

A

AZOCARMINE

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17
Q

plasma stain utilized also for deep staining acid-fast organisms, mitochondria, and differentiation of smooth muscles

A

BASIC FUCHSIN

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18
Q

Basic fuchsin are (5)

A
  1. CARBOL-FUCHSIN
  2. COLEMAN’S FEULGEN REAGENT
  3. SCHIFF’S REAGENT
  4. MALLORY’S FUCHSIN STAIN
  5. ALDEHYDE FUCHSIN (GOMORl’S STAIN)
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19
Q

used for staining hemoglobin

A

BENZIDINE

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20
Q

used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for staining diphtheria organisms

A

BISMARCK BROWN

21
Q

chromatin stain for fresh materials in smear preparations

A

CARMINE

21
Q

chromatin stain for fresh materials in smear preparations

A

CARMINE

22
Q

The most important component of carmine is _________

A

carminic acid

23
Q

carminic acid is also useful in _______ (2)

A
  1. industry
  2. analytical chemistry
24
Q

Carmine is used for determining the presence
of certain metal ions, such as _______

A

aluminum

25
Q

mordanted dye acting as a basic dye and staining acidic substances.

A

CARMALUM (MAYER’S) SOLUTION

26
Q

oxazine dye used as an alternative to iron hematoxylin nuclear stain

A

CELESTINE BLUE

27
Q

Celestine blue forms a strong staining lake with ______

A

iron alum

28
Q

best known as an indicator, but may be utilized as a stain for
axis cylinders in embryos

A

CONGO RED

29
Q

CONGO RED ; 4% aqueous solution stains (4)

A
  1. elastic fibers
  2. myelin
30
Q

Congo red is used to identify deposits of protein in tissue
called

A

amyloid

31
Q

commonly used in histology to stain nervous tissues; NISSL BODIES

A

CRESYL VIOLET

32
Q

nuclear or chromatin stain used for staining
amyloid in frozen sections and platelets in blood

A

CRYSTAL VIOLET

33
Q

Gentian violet is the staining solution formed by the mixture of ______________ (3)

A
  1. crystal violet
  2. methyl violet
  3. dexterin
34
Q

stains DNA, providing a fluorescent red-orange stain ; marker for apoptosis in cells populations

A

ETHIDIUM BROMIDE

35
Q

stains DNA, providing a fluorescent red-orange stain ; marker for apoptosis in cells populations

A

ETHIDIUM BROMIDE

36
Q

consists of a mixture of methylene-blue and eosin, and it is
used for staining blood to differentiate leukocytes

A

GIEMSA STAIN

37
Q

It also binds to some pathogens, including spirochetes (syphilis), trypanosomes (sleeping sickness and Chagas disease) and plasmodium (malarial parasites).

A

GIEMSA STAIN

38
Q

stain used for metallic impregnation

A

GOLD SUBLIMATE

39
Q

stain used for metallic impregnation

A

GOLD SUBLIMATE

40
Q

GOLD SUBLIMATE is made up of ___________(2)

A
  1. gold chloride
  2. mercuric chloride
41
Q

probably the oldest of all stains, originally used for microscopic study of starch granules

A

IODINE

42
Q

It is widely used for removal of mercuric fixative artefact pigments

A

IODINE

43
Q

is used to identify and differentiate bacteria

A

Gram’s Iodine

44
Q

brown solution that turns black in the presence of starches

A

Lugol’s solution or Lugol’s iodine

45
Q

_______ is also used as a mordant in Gram’s staining

A

Iodine

46
Q

used for demonstrating mitochondria during intravital
staining.

A

JANUS GREEN B

47
Q

a contrast stain for staining ascaris eggs and
erythrocytes, and as a bacterial spore stain

A

Malachite green

48
Q

it is also used both as a decolorizer and as a counterstain

A

Malachite green

MTAP 2 - STAINING ; FINALS (12 decks)

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