Other Stain

Question 1

mixture of picric acid and acid fuchsin for demonstration of connective tissues.

Answer 1

Acid Fuchsin-Picric Acid (Van Gieson’s Stain)

Question 2

used to stain collagen, smooth muscle, or
mitochondria

Answer 2

Acid Fuchsin (Masson Stain)

Question 3

In Van Gieson’s picro-fuchsin, acid fuchsin imparts its _______ to collagen fibers

Answer 3

red color

Question 4

basic acridine fluorochrome which permits
discrimination between dead and living cells

Answer 4

ACRIDINE ORANGE

Question 5

Acridine orange imparts _____ fluorescent in DNA

Answer 5

green

Question 6

Acridine orange imparts _____ fluorescent in RNA

Answer 6

Red

Question 7

used to demonstrate deposits of calcium salts and possible sites of phosphatase activities

Answer 7

ACRIDINE RED 3B

Question 8

a complex, water-soluble phthalocyanin dye, similar to chlorophyll

Answer 8

ALCIAN BLUE

Question 9

ALCIAN BLUE stains (3)

Answer 9

1. acid mucopolysaccharides
2. connective tissue
3. epithelial mucin

Question 10

Alcian blue is often combined with ______

Answer 10

PAS

Question 11

Alcian blue with PAS stains acidic mucins ______

Answer 11

blue

Question 12

Alcian blue with PAS stains neutral mucin _______

Answer 12

Red

Question 13

forms an orange-red lake with calcium at a pH of 4.2

Answer 13

ALIZARIN RED S

Question 14

ALIZARIN RED S works best with small amounts of calcium such as ___________

Answer 14

Michaelis-Gutman
bodies

Question 15

cytoplasmic stain used for counterstaining of epithelial
sections

Answer 15

ANILINE BLUE

Question 16

Nuclei are deep red; cytoplasm is a pale red

Answer 16

AZOCARMINE

Question 17

plasma stain utilized also for deep staining acid-fast organisms, mitochondria, and differentiation of smooth muscles

Answer 17

BASIC FUCHSIN

Question 18

Basic fuchsin are (5)

Answer 18

1. CARBOL-FUCHSIN
2. COLEMAN’S FEULGEN REAGENT
3. SCHIFF’S REAGENT
4. MALLORY’S FUCHSIN STAIN
5. ALDEHYDE FUCHSIN (GOMORl’S STAIN)

Question 19

used for staining hemoglobin

Answer 19

BENZIDINE

Question 20

used as a contrast stain for Gram’s technique, in acid fast and Papanicolau method, and for staining diphtheria organisms

Answer 20

BISMARCK BROWN

Question 21

chromatin stain for fresh materials in smear preparations

Answer 21

CARMINE

Question 21

chromatin stain for fresh materials in smear preparations

Answer 21

CARMINE

Question 22

The most important component of carmine is _________

Answer 22

carminic acid

Question 23

carminic acid is also useful in _______ (2)

Answer 23

1. industry
2. analytical chemistry

Question 24

Carmine is used for determining the presence of certain metal ions, such as _______

Answer 24

aluminum

Question 25

mordanted dye acting as a basic dye and staining acidic substances.

Answer 25

CARMALUM (MAYER'S) SOLUTION

Question 26

oxazine dye used as an alternative to iron hematoxylin nuclear stain

Answer 26

CELESTINE BLUE

Question 27

Celestine blue forms a strong staining lake with ______

Answer 27

iron alum

Question 28

best known as an indicator, but may be utilized as a stain for axis cylinders in embryos

Answer 28

CONGO RED

Question 29

CONGO RED ; 4% aqueous solution stains (4)

Answer 29

1. elastic fibers 2. myelin

Question 30

Congo red is used to identify deposits of protein in tissue called

Answer 30

amyloid

Question 31

commonly used in histology to stain nervous tissues; NISSL BODIES

Answer 31

CRESYL VIOLET

Question 32

nuclear or chromatin stain used for staining amyloid in frozen sections and platelets in blood

Answer 32

CRYSTAL VIOLET

Question 33

Gentian violet is the staining solution formed by the mixture of ______________ (3)

Answer 33

1. crystal violet 2. methyl violet 3. dexterin

Question 34

stains DNA, providing a fluorescent red-orange stain ; marker for apoptosis in cells populations

Answer 34

ETHIDIUM BROMIDE

Question 35

stains DNA, providing a fluorescent red-orange stain ; marker for apoptosis in cells populations

Answer 35

ETHIDIUM BROMIDE

Question 36

consists of a mixture of methylene-blue and eosin, and it is used for staining blood to differentiate leukocytes

Answer 36

GIEMSA STAIN

Question 37

It also binds to some pathogens, including spirochetes (syphilis), trypanosomes (sleeping sickness and Chagas disease) and plasmodium (malarial parasites).

Answer 37

GIEMSA STAIN

Question 38

stain used for metallic impregnation

Answer 38

GOLD SUBLIMATE

Question 39

stain used for metallic impregnation

Answer 39

GOLD SUBLIMATE

Question 40

GOLD SUBLIMATE is made up of ___________(2)

Answer 40

1. gold chloride 2. mercuric chloride

Question 41

probably the oldest of all stains, originally used for microscopic study of starch granules

Answer 41

IODINE

Question 42

It is widely used for removal of mercuric fixative artefact pigments

Answer 42

IODINE

Question 43

is used to identify and differentiate bacteria

Answer 43

Gram's Iodine

Question 44

brown solution that turns black in the presence of starches

Answer 44

Lugol's solution or Lugol's iodine

Question 45

_______ is also used as a mordant in Gram's staining

Answer 45

Iodine

Question 46

used for demonstrating mitochondria during intravital staining.

Answer 46

JANUS GREEN B

Question 47

a contrast stain for staining ascaris eggs and erythrocytes, and as a bacterial spore stain

Answer 47

Malachite green

Question 48

it is also used both as a decolorizer and as a counterstain

Answer 48

Malachite green