Streptococcus Flashcards
Gram (+) in cocci and chains
Streptococcus
Streptococcus motility
Non - motile
Streptococcus is non sporeforming: (T/F)?
True
Catalase (-), pinpoint colonies
Streptococcus
Selective medium of streptococcus
PEA (Phenyl Ethyl Alcohol Agar)
Classification based on antigens present on surface
Lancefield
Classification based on hemolytic reaction
Smith & Brown’s
Classification based on temperature
Bergey’s
Lancefield group A
S. pyogenes
Lancefield group B
S. agalactiae
Lancefield group C
S. dysagalactiae
S. equisimilus
S. equisimilis
S. equi
S. zooepidimicus
Lancefield group D
Enterococcus
E. faecalis
E. faecium
E. avium
E. ducans
Non enterococcus
S. bovis
S. equinus
Streptococcus that causes colon cancer
S. bovis
Alpha hemolytic
Streptococcus pneumoniae
Viridans
Group D
Beta hemolysis
Lancefield ABCD
Gamma hemolytic
Lancefield D
Bergey’s grouping: pus producing streptococcus
Pyogenic
Bergey’s grouping: used in making dairy products
Lactic
Bergey’s grouping: normal flora of the gut
Enterococcus
Bergey’s grouping: normal flora of upper respiratory tract
Viridans
Gram (+) diplococci
Lancet shaped
Dome shaped colonies
S. pneumoniae
Streptococcus catalase
(-)
White pinpoint colonies
Streptococcus
Virulence factor of (GA) S. pyogenes
M protein
O2 labile, antigenic, anerobic, lytic
Streptolysin O
O2 stable, non antigenic
Streptolysin S
Causes Scarlet fever
S. pyogenes
Toxin that causes Scarlet fever
Erythrogenic toxin
Bacitracin/Taxo A of S. pyogenes
Sensitive
Flesh eating bacteria
S. pyogenes
Causes impetigo
Pharyngitis
Rheumatic heart dxs
Rheumatic fever
Acute glomerulonephritis (AGN)
Erysipelas
Necrotizing fasciitis
S. pyogenes
Flesh eating bacteria
S. pyogenes
Beta hemolytic
Neonatal sepsis
Normal flora of vagina and upper respiratory tract
Pneumonia
Bacteremia
S. agalactiae
1 cause of neonatal meningitis
Resistant to Taxo A, P and SXT
(GB) S. agalactiae
Group of Streptococcus that rarely cause disease
Group C & D
Causes UTI
Pneumonia
Cellulitis
Lancefield Group C
Group C:
Bacitracin / SXT
(R)(S)
Test that differentiates Group D from other Strep groups
Bile Esculin Hydrolysis Test
Medium of Bile Esculin test
Bile Esculin Agar (BEA) contains 40% bile and esculin
Positive result for Bile Esculin
Blackening or brown ppt
(+) Group D streptococcus
Enterococci:
6.5% NaCl / PYR
(+)(+)
Non enterococci:
6.5% NaCl / PYR
(-)(-)
Positive result of PYR
Red
(+) Group D, Group A (S. pyogenes)
Meaning of CAMP test
Christine, Atkins, Munch, Peterson
Principle of CAMP test
CAMP factor enhances beta hemolysis
Positive result of CAMP test
Arrow head hemolysis
(+) S. agalactiae
(+) H. Pylori
(+) Listeria monocytogenes - no arrow head but w/ hemolysis
*Indicator: S. aureus
GA (S. pyogenes):
Bacitracin / SXT
(S)(R)
(+) PYRase
GB (S. agalactiae):
Bacitracin / SXT
(R)(R)
(+) CAMP
(+) Sodium hippurate
Group C:
Bacitracin / SXT
(R)(S)
GD (Enterococcus):
Bacitracin/SXT
(R)(R)
(+) BEA
(+) 6.5 NaCl
(+) PYRase
GD (Non enterococcus):
Bacitracin/SXT
(R)(R)
(+) BEA
Norma flora of orpharynx and nasopharynx
S. pneumoniae
Virulence factor of S. pneumoniae
Capsule
Alpha hemolytic
No Lancefield group
Causative agent of Lobar pneumonia
S. pneumoniae
1 community acquired pneumonia in the Ph
Lobar pneumonia
1 cause of adult bacterial meningitis
Most common cause of otitis media
S. pneumonia
Normal flora of upper respiratory, G.I and Genitourinary tract
Viridans
Alpha hemolytic
No Lancefield group
Cause of subacute bacterial endocarditis
Viridans
Causes dental caries
Viridans
S. pneumoniae:
Inulin fermentation / Bile solubility / Optochin (Taxo P) / Quellung
(+)(+)(S)(+)
Viridans:
Inulin fermentation / Bile solubility / Optochin (Taxo P) / Quellung
(-)(-)(R)(-)
Detects pneumococcal antibody; skin test for S. pneumoniae
Francis test
Detects erythrogenic antigen; skin test for S. pyogenes
Dick’s test
(+) Redness
Skin test for Scarlet fever
Schultz-Charlton test
(+) S. pyogenes - blanching of rash around injection site
Require cysteine for survival
Shows satelliting on agar
(+) Staph streak test
S. adjacens
Abiotrophia spp.
Pediococcus:
Vancomycin / Bile esc / PYR / NaCl / MRS / LAP
(R)(+)(-)(+)(-)(+)red
Leuconostoc:
Vancomycin / Bile esc / PYR / NaCl / MRS / LAP
(R)(+)(-)(+)(+)gas(N/A)
Aerococcus:
Vancomycin / Bile esc / PYR / NaCl / MRS / LAP
(S)(-/+)(-/+)(+)(N/A)(N/A)