Staphylococcus

Question 1

Virulence factor of S. Aureus

Answer 1

Protein A

Question 2

Staphylococcus hemolytic pattern

Answer 2

Beta hemolysis

Question 3

1 wound infection & #1 osteomyelitis

Answer 3

S. aureus

Question 4

Can cause boils and carbuncles

Answer 4

S. aureus

Question 5

Pigment of S. aureus

Answer 5

Cytochrome/Staphyloxanthin (golden yellow)

Question 6

S. aureus is inhibited by?

Answer 6

Bile

Question 7

MRSA grows in?

Answer 7

3°C with 2% NaCl

Question 8

Treatment for MRSA

Answer 8

Vancomycin

Question 9

S. aureus:
Coagulase / Acetoin / PYR

Answer 9

(+)(+)(-)

Question 10

S. Intermedius:
Coagulase / Acetoin / PYR

Answer 10

(+)(-)(+)

Question 11

Coagulase (+) staphylococcus

Answer 11

S. aureus
S. intermedius
S. hyicus
S. schleiferi

Question 12

S. hyicus
Coagulase / Acetoin / PYR

Answer 12

(+)(-)(-)

Question 13

S. schleiferi
Coagulase / Acetoin / PYR

Answer 13

(+)(+)(+)

Question 14

S. lugdunensis:
Coagulase / PYR

Answer 14

(+)(+)

Question 15

S. hemolyticus:
Hemolysis / Coagulase

Answer 15

(Beta)(-)

Question 16

DNAse (+) bacteria:
“SMASH VSS”

Answer 16

Serratia marcescens
Moraxella catarhalis
Aeromonas
Streptococcus pyogenes
Helicobacter pylori
Vibrio cholerae
S. maltophilia
S. aureus

Question 17

2 methods of DNAse test

Answer 17

a.
Toluidine blue (+): pink zone
Methyl green (-): clear zone
b.
HCl (+): clear
HCl (-): no clearing

Question 18

Differentiates coagulase (-) staphylococcus

Answer 18

Novobiocin test
*ID of S. saprophyticus

Question 19

Causes endocarditis (assoc. w/ Prosthetic Heart valve surgery)

Answer 19

S. Epidermidis

Question 20

Novobiocin (S)

Answer 20

S. Epidermidis

Question 21

Causes UTI in young women

Answer 21

S. saprophyticus

Question 22

Specimens for S. saprophyticus

Answer 22

Urine

Question 23

Novobiocin (R)
*less than 16mm

Answer 23

S. saprophyticus

Question 24

S. aureus:
Inhibits phagocytosis

Answer 24

Protein A

Question 25

S. aureus:
Converts fibrinogen to fibrin clot

Answer 25

Coagulase (+)

Question 26

S. aureus:
Dissolves fibrin clot

Answer 26

Staphylokinase

Question 27

S. aureus:
Degrades lipid (cellulitis, boils)
*fat splitting enzyme

Answer 27

Lipase

Question 28

S. aureus:
Degrades hyaluronic acid (cartilage)

Answer 28

Hyaluronidase

Question 29

S. aureus:
Degrades DNA

Answer 29

DNAse (+)

Question 30

S. aureus:
Destroys stratum granulosum (causes SSS)

Answer 30

Exfoliatin

Question 31

S. aureus:
Destroys PMNs, monocyte
*Aka. Panton Valentine factor

Answer 31

Leukocidin

Question 32

S. aureus:
Destroys RBC

Answer 32

Hemolysin

Question 33

S. aureus:
Causes food poisoning & septic shock

Answer 33

Enterotoxin

Question 34

1 cause of food poisoning in Ph

Answer 34

S. aureus (enterotoxin)

Question 35

Causes TSST -1
Beta lactamase (+)
Gelatinase (+)

Answer 35

S. aureus