Enterobactericiae Flashcards
IMVIC of Escherichia
(+)(+)(-)(-)
TSI of Escherichia
A/A - Lactose Fermenter
Decarboxylase of Escherichia: LOA
(+)(+)(-)
Escherichia in EMB agar
Greenish metallic sheen
Escherichia in XLD agar
Yellow colonies
No. 1 cause of UTI
No. 2 cause of infant meningitis
Escherichia
ETEC - TOXIGENIC is causative agent of?
Montezuma revenge / Travellers disease
Escherichia that produces cholera like toxin (rice watery stool)
ETEC
Escherichia that produces childhood diarrhea
ETEC
Causative agent of Montezuma’s revenge
Escherichia - ETEC
Escherichia that produces heat labile and heat stable enterotoxins
ETEC
Escherichia that causes Shigella like infection
EIEC - INVASIVE
Escherichia that causes dysentery (painful passage of stool)
EIEC - INVASIVE
Escherichia that cause stool w/ RBC, Mucus, WBC
EIEC - INVASIVE
Virulence test for EIEC
Sereny test
Escherichia that causes Infantile diarrhea
EPEC - PATHOGENIC
Non invasive, No toxin Escherichia
EPEC - PATHOGENIC
Escherichia causing watery diarrhea w/o RBC, WBC, and mucus
EPEC - PATHOGENIC
Aka. VTEC (Verotoxin Escherichia coli)
EHEC - HEMORRHAGIC
Most virulent strain of EHEC
E. coli 0157:H7
Escherichia that causes HUS (hemolytic uremic syndrome)
EHEC - HEMORRHAGIC
Escherichia that produces verotoxin (Shigella like toxin)
EHEC - HEMORRHAGIC
SMAC result of EHEC
(-)
Escherichia that causes acute and chronic diarrhea
EAEC - AGGREGATIVE
Escherichia that produces hemolysin
EAEC - AGGREGATIVE
Escherichia that produces ST like enterotoxin
EAEC - AGGREGATIVE
Escherichia motility
Non motile
IMVIC of Klebsiella
(-)(-)(+)(+)
TSI of Klebsiella
A/A
LIA of Klebsiella
K/K
Klebsiella:
Decarboxylase: LOA
(+)(-)(-)
except K. aerogenes (+)(+)(-)
Urease of Klebsiella
(+)
Klebsiella on MAC
Mucoid pink colonies
Only Klebsiella that is indole (+)
K. oxytoca
Klebsiella motility
Non motile
Enterobacter motility
Motile
IMVIC of Enterobacter
(-)(-)(+)(+)
Enterobacter:
TSI / LIA
A/A
K/K
Decarboxylase of Enterobacter: LOA
(+)(-)(-)
Enterobacter:
Urease
(-) except E. gergoviae
Bacteria known to cause upper respiratory tract infections
Klebsiella
Enterobacter
Serratia
Known to produce 3 enzymes.
What enzymes
Serratia
DNAse
Lipase
Gelatinase
Produces red pigment (prodigiosin)
Serratia
Serratia Decarboxylase: LOA
(+)(+)(-)
Serratia: Citrate
(+)
Sources of infection of Salmonella
Dairy, water, poultry pet house
Causes Typhoid fever, Enterocolitis and Enteric fever
Salmonella
Colonies of Salmonella in SSA
Black
All Salmonella are cultured in BGA except
S. typhii - inoculated in Bismuth Sulfite Agar (BSA)
Salmonella motility
Non motile
Most important spp. of Salmonella
S. typhii
Specimen for Salmonella:
1st week / 2nd week / best
1st: blood/urine
2nd: stool
Best: bone marrow
Salmonella causing Enterocolitis
S. typhimurium
Tube agglutination test for Salmonella
Widal test
Widal test:
O / H / Vi
O - Somatic ag
H - Flagellar ag
Vi - Capsular ag
Widal test: O (Somatic ag) titer
1:160 - active Salmonella infection
Widal test: H (Flagellar ag)
Past infection (IgM)
Widal test: Vi (capsular ag)
Carrier (IgG)
Common name of Shigella
Inert bacteria
Shigella motility
Non motile
Shigella on SSA
Colorless
Shigella:
TSI/LIA/LOA
K/A
K/A
(-)(-)(-)
Causes dysentery (stool w/ blood and mucus)
Shigella
Group type : O antigen
A/B/C/D
A - S. dysenteriae
B - S. flexnerii
C - S. boydii
D - S. sonnei
Shigella dysenteriae (Group A):
Catalase/ONPG/Mannitol
(-)(-)(-)
Shigella flexnerii & boydii (Group B & C):
Catalase/ONPG/Mannitol
(+)(-)(+)
Shigella sonnei (Group D):
Catalase/ONPG/Mannitol
(+)(+)(+)