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Enterobactericae (Biochem Test) Flashcards

1
Q

(EMB)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Eosin Y, Methylene blue
Lactose
Eosin Y, Methylene blue
Red/Purple
Colorless

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2
Q

(MAC)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Crystal violet, bile salt
Lactose
Neutral red
Red/pink
Colorless

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3
Q

(XLD)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Bile salt
Xylose, lactose, sucrose
Phenol red
Yellow
Red

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4
Q

(HEA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Bile salt
Salicin, lactose, sucrose
Bromthymol blue
Yellow
Green

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5
Q

(DCA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Bile salt
Lactose
Neutral red
Red/pink
Colorless

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6
Q

(SSA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Bile salt
Lactose
Neutral red
Red
Colorless:
*NLF with black center - Salmonella
* NLF without black center - Shigella

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7
Q

H2S Producers: SPACED

A

Salmonella
Proteus
Providencia
Arizona
Citrobacter freundii
Edwardsiella
(+): Blackening of medium

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8
Q

(BSA)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:

A

Brilliant green
Glucose
Bismuth sulfite
Black colonies (+): Salmonella typhii

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9
Q

(TCBS)
Inhibitor:
Carbon:
Indicator:
Lactose fermenter:
Non Lactose fermenter:

A

Bile salt
Sucrose
Bromthymol blue
Yellow
Green

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10
Q

(TSI)
Carbon:
pH Indicator:
H2s indicator:

A

Sucrose, Lactose, Glucose + Iron + Na thiosulfate
Phenol red (red to yellow)
FeSo4 (black; gas = splits medium)

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11
Q

TSI reactions:
A/A

A

Yellow/Yellow
lactose, sucrose, glucose fermented

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12
Q

TSI reaction
K/A

A

Red/Yellow
Glucose fermented

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13
Q

TSI: (A/A) - “EKEE + ACC”

A

E. coli
Klebsiella
Enterobacter aerogenes
Enterobacter cloacae

Arizona
Citrobacter freundii
Citrobacter diversus

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14
Q

TSI: (K/A) - “PPM + SSSY”

A

Proteus
Providencia
Morganella

All Salmonella except arizonae (LLF)
All Shigella except sonnei (LLF)
Serratia marcescens
Yersinia

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15
Q

TSI: (K/K) - “PAA”

A

Pseudomonas
Alcaligenes
Acinetobacter

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16
Q

Salmonella w/ small amount of H2S

A

Salmonella typhii

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17
Q

Salmonella w/ large amount of H2S

A

Salmonella typhimurium

18
Q

TSI reaction K/K

A

Red/Red
Non sugar fermenter

19
Q

LIA test principle

A

Detect to ability of bacteria to degrade acid lysine

20
Q

Indicator of LIA

A

Bromcresol purple

21
Q

LIA butt is indicator for?

A

Lysine decarboxylation

22
Q

LIA slant is indicator for?

A

Lysine deamination

23
Q

Lysine decarboxylation (butt)
(+)/(-)

A

(+) purple
(-) yellow

24
Q

Lysine deamination (slant)
(+)/(-) result

A

(+) red
(-) purple

25
Q

IMViC
(+) result of Indole

A

(+) red ring

26
Q

IMViC
(+)/(-) result for Methyl red

A

(+) red - E. coli
(+) yellow - Enterobacter cloacae

27
Q

IMViC
(+)/(-) result for Voges Proskauer

A

(+) red - “KESH” (Klebsiella, Enterobacter, Serratia, Hafnia)

(-) yellow

28
Q

Citrate utilization
(+)/(-) result

A

(+) blue - Klebsiella pneumoniae
(-) green - E. coli

29
Q

ACETATE/MALONATE/ACETAMIDE (AMA)
(+) result

A

A - E. coli
M - Salmonella
A - Pseudomonas

30
Q

ACETATE/MALONATE/ACETAMIDE (AMA)
(-) result

A

A - Shigella flexnerii
M - Shigella
A - Stenotrophomonas maltophilia

31
Q

Urease Test
(+)/(-) result

A

(+) red
(-) yellow

32
Q

Rapid Urease test - “PPM”

A

(+) after 2 hrs

Proteus
Providencia
Morganella

33
Q

Slow Urease - “CKEYS”

A

(+) after 4 hrs

Citrobacter
Klebsiella
Enterobacter gergovae
Yersinia
Serratia

34
Q

Lactose Fermentation
(Rapid) - “EKE”

A

Has 2 enzymes

Escherichia
Klebsiella
Enterobacter

35
Q

Enzymes on Rapid Lactose Fermenters

A

B galactosidase
Lactose permease

36
Q

Enzyme on Late lactose fermenters

A

B galactosidase only

37
Q

Lactose fermentation
(Late) - “S2 HYCS”

A

Salmonella arizonae
Shigella sonnei
Hafnia
Citrobacter

38
Q

Lactose fermentation
(Non) NLF

A

All Salmonella except arizonae
All Shigella except sonne
Yersinia
Serratia
Proteus
Providencia
Morganella
Other enterobactericiae

39
Q

ONPG detects?

A

B galactosidase for LLF
(+) ALL LATE LACTOSE FERMENTERS

40
Q

Decarboxylase uses 4 tubes: LOAC
(+) result

A

Lysine
Ornithine
Arginine
Control

(+) Purple/Yellow/Purple

41
Q

PAD (Phenylalanine deaminase)
(+)/(-) result

A

(+) green - “PPM”
Providencia
Proteus
Morganella

(-) E. coli

Bacteriology (13 decks)

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