Stool Flashcards
What is the family name of bacteria that includes mostly non-motile members and is often part of normal flora?
• Enterobacteriaceae
What can stool be used to detect?
• Stool can be used to detect food poisoning, diarrhea, and gastroenteritis.
What is the recommended media for inoculating a stool sample ?
• Inoculate the stool sample in the EMB, MAC, or SSA media.
What is the manner of streaking in the semi-quantitation technique for isolating bacteria?
• It involves inoculating four quadrants.
What is the purpose of the “PROCESS-INOCULATE” step in the cultivation of Stool samples?
• performed to isolate and grow “COLONIES” of microorganisms from the Stool sample on plated media
• allowing for subsequent biochemical analysis.
What is the purpose of the Biochem test in the Vitek system?
• Used to identify species and determine their susceptibility to specific processes,
• Ultimately leading to the separation of distinct species.
How does Vitek make biochemistry testing easier?
• Vitek makes biochemistry testing easier by requiring the pure isolation of species.
What can be the consequences of detecting GIT pathogens like salmonella and shigella, which are part of the Enterobacteriaceae family but not normal flora?
• It can lead to invasive infections and result in symptoms like bloody diarrhea.
How many serogroups are there under Shigella?
• There are four serogroups under Shigella: A, B, C, and D.
Which serogroup includes Shigella dysenteriae?
• Shigella dysenteriae belongs to serogroup A.
Which serogroup includes Shigella flexneri?
• Shigella flexneri is part of serogroup B.
Which serogroup includes Shigella boydi?
• Shigella boydi is associated with serogroup C.
What characterizes serogroup D in Shigella?
• Serogroup D in Shigella is represented by Shigella sonnei,
• And it is known as a late lactose fermenter.
What alternative sample can be used for testing if stool collection is not possible?
• A rectal swab can be used as an alternative for testing when stool collection is not possible.
How many quadrants are typically streaked on plated media when assessing stool samples?
• Four quadrants are often streaked on plated media when evaluating stool samples.
