Cerebrospinal fluid (CSF)

Question 1

Why is cerebrospinal fluid (CSF) cultured in medical testing?

Answer 1

• Cerebrospinal fluid (CSF) is cultured to rule out meningitis

Question 2

What is the recommended method for collecting cerebrospinal fluid (CSF)?

Answer 2

• Collect CSF in 3 tubes via a lumbar tap (invasive procedure).

Question 3

What should be done with the cerebrospinal fluid (CSF) collected in the #2 tube?

Answer 3

• The Cerebrospinal fluid (CSF) in the #2 tube should be micro-centrifuged.

Question 4

What is the purpose of centrifuging the CSF, and what should be done afterward?

Answer 4

• Centrifuging the CSF helps remove supernatant and isolate sediments for further analysis.

Question 5

What is the recommended storage temperature for Cerebrospinal fluid (CSF)?

Answer 5

• The recommended storage temperature for CSF is 37°C.

Question 6

What should Cerebrospinal fluid (CSF) be used for when preparing smears and cultures?

Answer 6

• Cerebrospinal fluid (CSF) should be used for smear preparation and culture by using the sediments.

Question 7

What type of samples should be used for bacterial recovery in cerebrospinal fluid (CSF) analysis?

Answer 7

• Bacterial recovery in Cerebrospinal fluid (CSF) analysis should be performed using sediments.

Question 8

What is the number one cause of neonatal meningitis in relation to cerebrospinal fluid (CSF)?

Answer 8

S. Agalactiae

Question 9

Which age group of children is commonly affected by Haemophilus influenzae serotype b in relation to cerebrospinal fluid (CSF)?

Answer 9

• Children under 5 years old are commonly affected

Question 10

What is the primary causative agent of adult meningitis, over what age?

Answer 10

• Streptococcus pneumoniae is the primary causative agent of meningitis in adults over 29 years old.

Question 11

Which bacterium is associated with bacterial meningitis in individuals aged 5 to 29 years old, according to the statement about CSF?

Answer 11

• Neisseria meningitidis (N. meningitidis) is associated with bacterial meningitis in individuals aged 5 to 29 years old.

Question 12

In cases of meningitis occurring in the elderly or individuals with compromised immune systems, what bacterium is commonly involved?

Answer 12

• Listeria monocytogenes (L. monocytogenes)
• Commonly involved in meningitis in the elderly or immunocompromised individuals.

Question 13

What should be added to cerebrospinal fluid (CSF) collected from a shunt to facilitate the growth of FACULTATIVE anaerobes and streak aerobes/anaerobes?

Answer 13

• Thioglycolate can be added to the CSF to achieve this purpose.