Sterilization and Disinfection Flashcards
Disinfection is defined as the reduction or destruction of ______ pathogenic microorganisms on ______ (nonliving) objects.
most, inanimate
Disinfectant is defined as chemical agents that reduce ______ _____ on ______ objects.
microbial load, inanimate
is a chemical agent
Sterilization is defined as the elimination of ____ microbes on an inanimate object
all
Antiseptic is defined as the chemical agents that ______ microbial load or inhibit ______ of microbes on _____/_____ objects.
reduce, growth, living, animate
e.g. what we use to clean a patient during surgery
Sterility is defined as the absence of _____ forms of microbial life only _____ objects
all, inanimate
Which words should be paired together?
Examples of low level contamination
Don’t require disinfection unless they are really dirty
• Reception desks
• Offices
• Walls
• Doors
Examples of high level contamination
Minimum thorough cleaning
Drying agent followed by a low level disinfectant
• Kennels/housing
• Exam tables
• Operating tables/floors
When selecting a disinfectant, what should you consider?
- What level of disinfection is required?
• critical
• semi-critical
• non-critical - Is it compatible to the item you want to disinfect?
- Is it user friendly?
- Is it cost effective?
“Critical” level of sterility and disinfection applies to equipment or implants entering ____, ____ tissue, ____, or ______. This includes (4).
body, sterile, cavity, bloodstream
“Semicritical” level of sterility and disinfection applies to equipment that comes in contact with _____ or _______ ______ _______ penetrating body or cavity. This includes (6).
skin, mucous, membranes, without
In terms of semicritical sterility and disinfection, items must be _____ and ______ to reduce microorganisms but since sterility is ___ maintained during procedure sterility isn’t _____. _____
sterilization is common though.
cleaned, disinfected, not, required, Terminal
“Non-critical” level of sterility and disinfection applies to equipment that comes in contact with _____ skin or mucous membranes but not _____ associated with surgery. This includes (7).
intact, directly
In terms of non-critical sterility and disinfection, equipment should be _______ but do not require
specific handling ________ patients. Should be _______ cleaned when done for the day.
disinfected, between, terminally
Common disinfectants
Do not memorize this slide
Just know the compounds
What microbes does Isopropyl Alcohol (IPA) kill?
Bacteria, fungi, viruses, NOT spore.
What microbes does Povidone-iodine (PVI) kill?
Bacteria (fast), fungi, viruses, spores
What microbes does Chlorhexidine Gluconate
(CHG) kill?
Bacteria (fast), fungi (poor), viruses (poor), NOT spores
What microbes does Alcohol-based solutions kill?
Bacteria, +/- = fungi, viruses, spores
Isoprpyl Alcohol is considered to be both a _______ and an _______.
disinfectant, antiseptic
IPA is _________ ( _________ at lower
concentrations) and kills many _____ and _____
bactericidal, bacteriostatic, fungi, viruses
Describe IPA’s residual activity?
Minimal (evaporates)
IPA is less effective than ?
PVI or CHG
IPA is not typically used as a ______ _______ agent.
cold sterilization
IPA is helpful in removing excess ____ on the ____ prior to surgery
lipids, skin
Povidone-iodine (PVI) is considered to be both a _______ and ______.
disinfectant, antiseptic
Describe the activity of PVI?
• Bactericidal
• Fungicidal
• Effective against viruses
• Sporocidal (15 min contact time)
Describe the residual activity of PVI.
Minimal; Effectiveness decreased in presence of organic material
The concentration of PVI varies in accordance to its ____.
• For wound management: ___ to ___% solution
• For surgical prep: ___% scrub/solution
use, 0.1, 1, 10
Chlorhexidine Gluconate (CHG) is an ______.
Antiseptic
Describe Chlorhexidine Gluconate (CHG) activity.
• Bactericidal (rapid; quicker with GM + > GM-)
• Minimal activity against viruses and fungi
• Not sporicidal
Describe CHG’s residual activity
• Residual activity due to _____ binding
• Not inactivated by organic _____, _______, or _____ (so even if you have a dirty patient, it will still be actively working)
keratin, debris, alcohol, or soaps
For CHG, concentration varies according to its use:
• For wound management: _____% solution
• For surgical prep: ___% scrub/solution
0.05, 4
Alcohol based solutions are considered to be _______.
antiseptics
Alcohol based solutions have a superior ________ activity than PVI, CHG, or IPA alone
antimicrobial
The residual activity of alcohol based solutions is ?
More than alcohol alone
The usage of alcohol based solutions is a ____-step process (“_____”, single application and let dry)
• ChloraPrep (2% CHG + 70% IPA)
• ACTIPREP (83% Ethanol + Zinc Pyrithione)
• DuraPrep (PVI + 74% IPA
one, scrubless
Sterilization is an ______ state, there is no such thing as almost or sort of sterile. It either is or isn’t.
absolute