Sterilisation and disinfection Flashcards

1
Q

What is disinfection?

A

The destruction or removal of all or nearly all pathogens, but not all microorganisms

This process may not be absolute & may result in only a reduction in the number of potentially harmful microorganisms

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2
Q

What is sterilisation?

A

The destruction or removal of all microorganisms

This is an ‘absolute’ & at the end of a sterilisation process, no viable microorganisms are present

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3
Q

What is decontamination?

A

Decontamination is a combination of processes carried out to render a contaminated reusable medical device safe to use again

Not a sterility absolute; it depends upon the nature of the equipment & the use characteristics for the types of procedure for which they are required

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4
Q

How are decontamination requirements classified?

A

By procedure risk

  1. critical risk:
    - Procedure Type (use characteristics): Percutaneous entry into tissue, body cavity or vascular system (surgical access)
    - Level of Decontamination Required: Preoperative instrument sterility required
  2. Semi critical:
    - Procedure Type (use characteristics): Surface contact with diseased or non-intact skin or with mucous membranes
    -Level of Decontamination Required: Sterilisation preferred but minimum of preoperative disinfection required if sterilisation not possible
  3. Non-critical:
    -Procedure Type (use characteristics): Contact with healthy intact skin
    -Level of Decontamination Required: Disinfection preferred as a minimum. Item visibly cleaned by standardised process
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5
Q

Can light guns be sterilised?

A

No- so it needs to be barrier wrapped

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6
Q

What is cross-infection?

A

The transmission of a microorganism (infection) from one place/person to another in the same healthcare environment

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7
Q

What does the cycle of infection look like?

A
  1. Infectious agent: bacteria, viruses, fungi, protozoa, helminths etc.
  2. Reservoir: people, equipment, water
  3. Portals of exit: excretions, secretions, droplets, skin
  4. Means of transmission: direct contact/fomites, injection/ingestion, airborne, aerosols
  5. Portal of entry: broken skin, mucous membrane, gastrointestinal/respiratory/urinary tract
  6. Susceptible host: neonates, diabetics, immunosuppressed, cardio pulmonary disease

slide 15

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8
Q

What types of infectious agents are transmissible during healthcare procedures?

A

Bacteria (MRSA, MSSA, etc.)

Viruses (HBV, HCV, HIV, etc.)

Fungi (C. alb)

Prions (vCJD)

Protozoa

Helminths

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9
Q

What can act as a microorganism ‘reservoir’?

A

areas where microorganisms can reside and proliferate - within people, environments, instruments and equipment

Non porous-load ‘solid’ instruments (scaler)

Porous-load instruments & equipment
look at slide 19

cfu= colony forming unit

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10
Q

What is the commonest & most important area of cross contamination?

A

Our hands

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11
Q

How do microorganisms get from the instruments that we use into the patient?

A

Aerosol, inject, splatter, contact

Everything must be sterile!

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12
Q

What are the mechanisms of cross contamination (infection)?

A

Direct contact: infectious agent transmission from same or different person

Indirect contact: via instruments or equipment

Inhaled/airborne: DUWL’s, sneezing, coughing, etc.

Ingested: food & water hygiene

Vector: clothing, towels, insects, animal contact, etc.

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13
Q

What are the different portal of entry for the microorganisms?

A

Gingival margin, possible cavities, surgical wounds, area of infection, area of non intact mucosal skin, area of non healing bone, etc.

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14
Q

How are medical devices decontaminated?

A

Disinfected by chemical, mechanical or thermal means

Sterilised by either irradiation, chemical or thermal means

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15
Q

What does the decontamination cycle look like?

A
  1. use
  2. transport
  3. clean
  4. disinfect
  5. inspection
  6. package
  7. sterilisation
  8. transport
  9. store
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16
Q

What are the modifying factors for effective disinfection & sterilisation?

A

Nature of microorganisms: virus vs spores

Level of contamination: bio-load

Environmental conditions: moisture, temperature

Nature of equipment to be decontaminated: material, complexity

E.g. the 3 in 1 can’t be sterilised so it has to be barrier-covered

17
Q

what are the 4 cycle stages of automatic washer-disinfectors?

A
  1. Cool rinse at 35 degrees

2.Hot wash at 55 degrees

3.Thermal disinfect at 93 degrees

4.Forced air-drying

slide

18
Q

How efficient are automatic washer-disinfectors?

A

Manual cleaning: 35% efficiency

Ultrasonic cleaner: 55% efficiency

Domestic dishwasher: 70% efficiency

Medical washer disinfector: 95%+ efficiency

19
Q

What are the different types of irradiation?

A

Non-ionising: UV light

Ionising: X-rays, gamma rays, electron beams

20
Q

What different chemical sterilisation methods are used?

A

Liquid: glutaraldehyde, formaldehyde, hydrogen peroxide, paracetic acid

Gas: ethylene oxide, nitrogen dioxide, ozone

21
Q

How do you thermally sterilise?

A

Direct heat: instrument ‘flaming’, incineration

Indirect heat: particulate= glass bead, dry= hot air ‘oven’,
moist= steam ‘autoclave’

22
Q

What are the parameters for autoclave sterilisation?

A

temp. no higher than 134-137 degrees or else it would damage instrument

Time = 3 mins needed for sterilisation at 134-137 degrees but 18 mins at 121 degrees

Pressure = 2.2-2.5 bar

slide 37

23
Q

What are the different autoclave steriliser types?

A

N type
B type
S type

24
Q

what is an n type Autoclave Sterilizer?

A

AKA: non-vacuum, downward displacement

Used for solid, non-porous and unwrapped, un-pouched instruments only

25
Q

what is a b type Autoclave Sterilizer?

A

AKA: Vacuum, forced air removal

Used for wrapped, pouched, porous (air-retentive) materials and instruments

26
Q

what is a s type Autoclave Sterilizer?

A

Special autoclaves for specific decontamination purposes e.g. endoscopes & dental hand piece sterilisation