Staphylococcus

Question 1

Where can Staphylococci be found

Answer 1

occur worldwide, commensals on skin, muc. memb.

food, plants, soil, water

Question 2

Satphyl. can also sometime be found in the upper resp. tract and the lower Ug tract T/F

Answer 2

T

Question 3

Morphology of S

Answer 3

Coccus 1 micrometer in diameter

form irregular clusters - bunch of grapes

Question 4

S are flagellated Bb T/F

Answer 4

F

Question 5

S are spore forming Bb T/F

Answer 5

F

Question 6

S are non capsulated Bb T/F

Answer 6

T

Question 7

S have flagella and a capsule while exposed to CO2 T/f

Answer 7

F

Question 8

S are motile Bb despite having no flagella T/F

Answer 8

F

Question 9

Which agar do S need

Answer 9

simple nutrient agar

Question 10

Do S need oxygen

Answer 10

not necessarily

Question 11

What are S in regard to oxygen

Answer 11

facultative anaerobs

Question 12

How can S be categorized

Answer 12

coagulase +

coagulase -

Question 13

On which medium can S be cultured

Answer 13

simple nutrient agar

Question 14

Do S from pigments

Answer 14

yes

Question 15

Which pigments do S form

Answer 15

white , golden (Ru,Ho strains)

Question 16

How can you culture S selectively

Answer 16

10%NaCl - mannitol - phenolred agar
OR
Na tellurite - glycine - pyruvic acid - eggyolk - Baird parker agar

Question 17

What do S colonies look like on Baird parker agar

Answer 17

black

Question 18

C O

Answer 18

catalase +

oxidase -

Question 19

O F test

Answer 19

fermentative

Question 20

Characterise coagulase + S

Answer 20

• ec enzymes
• toxins
• haemolysis
• mannitol +
• fac. path

Question 21

Characterizecoagulase - S

Answer 21

• less ec enzymes
• usually no toxin prod
• no haemolysis
• mannitol -
• generally saprophytes

Question 22

Name the Coag. + Species

Answer 22

• S.aureus ssp. aureus
• S.aureus ssp. anaerobicus
• S.pseudointermedius

Question 23

Name the Coag.- Species

Answer 23

• S.hyicus
• S.epidermidis
• S.haemolyticus
• S.gallinarum
• S.equorum
• S.Felis

Question 24

What does S.aureus ssp aureus cause

Answer 24

lucal suppuration, abscesses, infections after surgery

Question 25

What does S.aureus ssp aureus cause in what species

Answer 25

Poultry: arthritis
Ov,Ru,Goat,Su,Eq: mastitis 
Su: Metritis
Ov, goat, Rabbit, poultry: dermatitis
Ru,Su: udder impetigo
Young lambs, rabbits turkey: septicaemia

Question 26

What causes MOREL disease or caseous lymphadenitis of sheep

Answer 26

S.aureus ssp. anaerobicus

Question 27

Pyoderma, pyometra, mastitis, endometritis of Dogs and cats is caused by which Bb

Answer 27

S.pseudointermedius

Question 28

What does S.hyicus cause

Answer 28

exudative epidermitis (greasy pig disease) under 3 months of age

Question 29

What do the rest of the Coagulase - S cause

Answer 29

dermatitis, local suppuration

Question 30

How to perform Gram staining

Answer 30

• dry sample
• crystal violet 3-5 min
• wash
• lugol 1-1,5 min
• wash
• 96% alc some drops
• water immediately
• fuchsin 0,5-1 min
• wash
• dry
• enjoy