Mycobacterium Flashcards
What are M
intracellular Bb - survive in infected cell
Where can the obl. pathogens only be found
in infected cells
Where can the fac. path and saprphytes be found
in the environment, soil, water, plants
What is the morph of M
rods, coccoid difference in cell structure in different species
What’s the resistance like
excellent
Staining
gram + but gram often doesn’T work
Ziehl Neelsen +
O2
obl aerobic
Culture
broth: Henley, Sauton
solid: egg, serum, agar
long inc.
Antigens
a lot Glycolipids, lipoproteins: -shared -cross reactions tuberculin
Special test for tuberculin
PPD (purified protein derivate) basically a transdermal injection of tuberculin and if a person had been subjected to it before AB reaction would show
!Name all the obl. pathogens in M and their possible hosts! (10)
M. tuberculosis - Ho, Su, Ca,Fe,Parrot, RU M.africanum - Ho M.Bovis - Ru, ru, Su, Ca, Fe, Ho M.caprae - Ru, ru, Su, Ho M.microti - rodents M.avium ssp avium - birds M.avium ssp paratuberculosis - Ru, ru M.avium ssp silvaticum - Av, Ru M.leprae - Ho M.lepraemurium - Ho, mouse
How are M. leprae, lepraemurium special
well they cause and also they’re parathrophic meaning they need living cells and cannot be cultured on synthetic medium
How do ZN + Bb stain
red
How do ZN - Bb stain
blue
What is the order of ZN staining
fixed - carbol-fuchsin - acid and alkohol - methylene blue