Stamcellegenetik Flashcards

1
Q

Turnover

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Pluripotente celler

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Totipotente celler

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Multipotente celler

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Oligopotente celler

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Unipotente celler

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Genetisk behandling af recessiv loss-of-function mutation

A

Tilførsel af nyt gen vha. genterapi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Genetisk behandling af dominant gain-of-function mutation

A

Reperation af mutationer (konventionel metode) eller knockout (CRISPR)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In vivo

A

I kroppen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Ex vivo

A

Udenfor kroppen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Retrovirale vektorer

A

Integreres i genomet. Bruges til behandling af stamceller, der laver meget celledeling. Risiko for aktivere proto-onkogener

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Lentivariale vektorer

A

Integreres i genomet. Bruges til behandling af stamceller, der laver meget celledeling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

AAV adeno associeret virus

A

Integreres ikke i genomet. Bruges til in vivo behandling af celler, der ikke laver meget celle deling.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Serotype

A

Den virale vektors “kappe”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

NHEJ

A

-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

HDR

A

-

17
Q

Cas9

A

-

18
Q

sgRNA

A

Guide RNA…

19
Q

PAM-sekvens

A

-

20
Q

Geneditering

A

-

21
Q

In vivo

A

I kroppen

22
Q

Ex vivo

A

Udenfor kroppen

23
Q

In vitro

A

I cellekultur

24
Q

Episomal

A

Når virus’ DNA ikke integreres i værtscellens genom. Gælder AAV vektor

25
Q

I celler/væv, der deler meget, genediteres typisk i

A

Stamceller

26
Q

I celler/væv, der ikke deler meget, genediteres typisk i

A

Selve vævet og episomalt

27
Q

Off-targets

A

-

Sker når RNA, DNA eller protein indsættes?