Stains and cultures Flashcards
Organisms that don’t stain well (6)
and why?
- treponema (too thin)
- mycobacteria (high lipid content in cell wall… need acid-fast)
- Mycoplasma (no cell wall)
- Legionella pneumophila (intracellular)
- Rickettsia (intracellular)
- Chlamydia (
“These Microbes May Lack Real Color”
Types of stains (6)
- gram
- giemsa
- PAS (periodic acid schiff)
- Ziehl-neelsen (carbol fuchsin)
- india ink
- silver stain
Giemsa stain bugs (5)
- Chlamydia
- Rickettsiae
- Trypanosomes
- Plasmodium
- Borrelia
Boring Plasma “Gems” are found intracellularly
PAS (periodic acid-Schiff)
- what does it stain
- used for what
stains glycogen, mucopolysacs
used to dx Whipple disease (Troperyma whipplei)
PASs the glycogen
ziehl-neelsen (carbol fucshin)
acid-fast organisms
- nocardia, mycobacterium
india ink
cryptococcus neoformans
silver stain (3) bugs
- fungi (pneumocystis)
- legionella
- helicobacter pylori
What media needed for H-flu?
chocolate agar w/factors V (NAD) and X (hematin)
What media needed for neisseria gonorrhoeae and meningitidis
Thayer-Martin (or VPN) media
Vanco: inhibits GP growth
Polymyxin: inhibits GN growth (except neisseria)
Nystatin: inhibits fungi growth
To connect to Neisseria, please use your VPN client
What media needed for B. pertussis
Bordet-Gengou (potato) agar
“Bordet for Bordetella”
What media needed for C. diphtheriae
Tellurite agar
Loffler medium
What media needed for M tuberculosis
Lowenstein-Jensen agar
What media needed for M pneumoniae
Eaton agar, requires cholesterol
What media needed for lactose-fermenting enterics
MacConkey agar - if fermenting –> pink
What media needed for legionella
charcoal yeast extract agar buffered w/cysteine and iron
