Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

Histopathology > STAINS > Flashcards

STAINS Flashcards

1
Q

most important and most commonly used for routine histologic studies

A

Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

mainly used for staining elastic fibers

A

Orcein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

A vegetable dye extracted from lichens

A

Orcein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What color does Orcein produces when treated with ammonia?

A

Blue or Violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Cochineal + Alum

A

Carmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Used as a powerful chromatin and nuclear stain for fresh material and smear prep

A

Carmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Cochineal + Picric acid

A

Picrocarmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

used on neuropathological studies

A

Picrocarmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Cochineal + Aluminum chloride

A

Best carmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

used for the demonstration of glycogen

A

Best carmine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A plant with orange stigmas yielding a dye

A

Saffron

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Oxidizing agents used to accelerate Hematoxylin:

  1. Mercuric oxide
  2. Sodium iodate
    3.Potassium permanganate
  3. Calcium hypochlorite
  4. Hydrogen peroxide
  5. Sodium hypochlorite
  6. Aluminum
  7. Copper
A

1, 2, 3, 4, 5

  1. Mercuric oxide
  2. Sodium iodate
    3.Potassium permanganate
  3. Calcium hypochlorite
  4. Hydrogen peroxide
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

The complex of stain and mordant is called:

A

Lake

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

most commonly used mordant that gives a blue lake

A

Aluminum / Alum salts

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

When used as mordant, this causes intense blue-black

A

Ferric salt

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Blueing agents

A
  1. 1% alcoholic ammonia or ammonia water
  2. 1% aqueous lithium carbonate
  3. Scott’s tap water / Scott’s tap water substitute
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Oxidizing agent used in Harris’ hematoxylin

A

Mercuric oxide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Oxidizing / Ripening agent used in Mayer’s/Ehrlich’s hematoxylin

A

Sodium iodate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Oxidizing agent used in Coles hematoxylin

A

Alcoholic iodine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Recommended for routine purposes especially used in sequence with Celestine blue

A

Coles Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Alum Hematoxylins

A

Mayer’s
Ehrlich’s
Delafield’s
Gill’s
Harris’
Cole’s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Iron Hematoxylins

A

Weigert’s
Heidenhain’s

23
Q

Used for the demonstration of spermatogenesis.

A

Copper hematoxylin

24
Q

A red acid dye used for staining cytoplasm differentially

25
Most commonly used Eosin
Eosin Y
26
Examples of Eosin
Eosin Y Eosin B Eosin S
27
Addition of ____ makes eosin staining more intense and selective
0.5 mL Glacial acetic
28
a special fixative used in frozen section which serves to localize antigens and hydrolytic enzymes
Formol Calcium
29
also used for preservation of lipids
Formol Calcium
30
H&E Color Reaction **Cytoplasm & proteins in edema fluid**
Pale pink
31
H&E Color Reaction **Decalcified bone, Osteoid & Collagen**
Pink
32
H&E Color Reaction **Muscle Fiber**
Deep pink
33
H&E Color Reaction **RBC, Eosinophil granules & Kerartin**
Bright orange to Red
34
H&E Color Reaction **Calcium & Calcified Bone**
Purplish blue
35
H&E Color Reaction **Plasma cells, Osteoblast & Basophilic cytoplasm**
Purplish pink
36
H&E Color Reaction **Karyosome**
Dark blue
37
H&E Color Reaction **Nucleus**
Blue to Blue-black
38
H&E Color Reaction **Cartilage**
Light blue to Dark blue
39
Stains used in Papanicolaou stain
Hematoxylin OG-6 Eosine Azure
40
for staining nuclear structures
Hematoxylin
41
for staining cytoplasm of mature cells
Orange Green-6
42
for staining cytoplasm of immature cells
Eosin Azure
43
Composition Eosin Azure
Eosin Bismarck brown Lithium carbonate PTA Light green stain
44
Chief solvents used for cells
1. Water 2. Alcohol 3. Phenol 4. Aniline water
45
Appearance of **Malarial pigments** in tissue
Brownish black intercellular granules
46
Removal of **Malarial pigments**
Alcoholic picric acid
47
Appearance of **Chromic fixatives** pigments in tissue
Fine yellow-brown deposits
48
Removal of **Chromic fixatives **
Acid alcohol
49
**Black granular** deposits in tissue
Mercuric chloride pigments
50
Removal of Mercuric chloride pigments
Alcoholic iodine
51
**Black precipitate** appearance in tissue
Osmium tetroxide fixatives
52
Removal of black precipitate in Osmium tetroxide fixatives pigments
Cold water
53
**Extracellular brownish black granules** apperance in tissue is caused by?
Acid formaldehyde hematin (Formol heme pigment)
54
Removal of Acid formaldehyde hematin pigments
Alcoholic picric acid or Alcoholic KOH (1% KOH in 80% Alcohol)

Histopathology (5 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions