MOUNTING

Question 1

A syrupy fluid applied between the section and the coverslip after staining, setting the section firmly, preventing the movement of the coverslip.

Answer 1

Mounting Medium

Question 2

To protect stained section from physical injury and from bleaching or deterioration of the stain as a result of oxidation.

Answer 2

Mounting Medium / Mountant

Question 3

For best result stained tissue sections must be impregnated by a transparent medium with a refractive index of

Answer 3

1.518

Question 4

TRUE OR FALSE

Mounting medium should be colorless and transparent.

Answer 4

TRUE

Question 5

TRUE OR FALSE

It should have a refractive index
close to that of diamond

Answer 5

FALSE

It should have a refractive index
close to that of GLASS

Question 6

TRUE OR FALSE
It should be freely miscible with xylene and toluene.

Answer 6

TRUE

Question 7

TRUE OR FALSE

It should not dry to a non-stick consistency and soften relatively quickly

Answer 7

FALSE

It should not dry to a non-stick consistency and harden relatively quickly

Question 8

TRUE OR FALSE

It should not cause shrinkage and distortion of tissues

Answer 8

TRUE

Question 11

Designed to mount water-miscible preparations directly from water in cases where the stain is removed or decolorized with alcohol or xyelene

Answer 11

Aqueous / Water soluble mountants

Question 12

Low refractive index

Answer 12

Water

Question 13

good only for temporary mounting

Answer 13

Water

Question 14

also used as a preservative

Answer 14

Glycerin

Question 15

has high index of refraction

Answer 15

Glycerin

Question 16

Refractive Index of Glycerin

Answer 16

1.47

Question 17

does not solidify upon storage

Answer 17

Farrant’s medium

Question 18

RI of Gum syrup

Answer 18

1.43

(i love Gum)

Question 19

used for methylene blue stained nerve preparations and as general purpose aqueous mountant

Answer 19

Apathy’s medium

Question 20

recommended for mounting frozen sections from water or paraffin sections which require dehydrating and clearing

Answer 20

Brun’s fluid

Question 21

used for preparations that have beeen dehydrated and cleared in xyelene or toluene and are commended for majority of staining methods

Answer 21

Resinous mountants

Question 22

natural resin extracted from the Canadian Tree, Abus balsamea

Answer 22

Canada Balsam

Question 23

recommended for whole mounts and for thick sections because it does not shrink much

Answer 23

Canada Balsam

Question 24

Miscible with xyelene and is quite expensive

Answer 24

Canada Balsam

Question 25

RI of Canada Balsam

Answer 25

1.524

Question 26

recommended for small tissue sections

Answer 26

Dibutyphthalate polystrene xylene (DPX)

Question 27

RI of DPX

Answer 27

1.532

Question 28

RI of XAM

Answer 28

1.52

Question 29

synthetic resin mixture in xylene in pale yellow or colorless solution

Answer 29

XAM

Question 30

RI of Clarite

Answer 30

1.544

Question 31

synthetic resin hich is soluble in xylene

Answer 31

Clarite

Question 32

PERMANENT MOUNTING MEDIA

Answer 32

1. Canada Balsam
2. D.P.X
3. Clarite
4. Eukitt
5. Entellan

Question 33

this dissolves out the essential staining

Answer 33

Xylol

Question 34

For some preparations, it is imperative not to use the permanent _ mounting media

Answer 34

Xylol-resin

this dissolves out the essential staining

Question 35

SEMI-PERMANENT AND TEMPORARY MOUNTING MEDIA

Answer 35

1. Water
2. Glycerin
3. Mineral Oil
4. Glycerin jelly
5. Von Apathy’s Gum syrup medium
6. Farrant’s medium
7. Levulose (Fructose) Syrup