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Histopathology > COUNTERSTAINS > Flashcards

COUNTERSTAINS Flashcards

1
Q

RED cytoplasmic stains

A

Eosin Y
Eosin B
Phloxine B
Rose Bengal

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2
Q

YELLOW cytoplasmic stains

A

Picric acid
Orange G

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3
Q

GREEN cytoplasmic stains

A

Light green SF
Lissamine green

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4
Q

RED nuclear stain

A

Carmine
Neutral Red
Safranin

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5
Q

BLUE nuclear stain

A

Methylene blue
Toluidine blue
Celestine blue
Hematoxylin

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6
Q

simplest connective tissue stain

A

Van Gieson’s

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7
Q

uses acid stain; no color

A

Masson’s trichrome

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8
Q

can also stain hyaline fibrils, amyloid and smooth muscles

A

Mallory’s Aniline blue

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9
Q

for Renal Glomerular basement membraine staining

A

Azocarmine

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10
Q

ELASTIC FIBERS STAINS

A
  1. Weigert’s Elastic tissue stain (dark blue/blue black)
  2. Taenzer-Unna Orcein Method (dark brown)
  3. Verhoeff’s stain
  4. Masson’s trichrome
  5. Krajian’s (bright blue)
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11
Q

RETICULUM STAINS

A
  1. Silver impregnation technique
  2. PAS
  3. Bielschowsky
  4. Periodic acid leucofuchsin technique
  5. Gold method
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12
Q

Carbohydrate stain that produce magenta red color

A

PAS

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13
Q

most common method used for the demonstration of glomerular basement membrane of kidney

A

PAS Technique

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14
Q

PAS positive substances

A
  • Carbohydrates
  • Phopholipids
  • Glycolipids
  • Glycoproteins
  • Unsaturated lipids
  • Mucoproteins

(CAPGGUM)

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15
Q

GLYCOGEN STAINS

A

1.PAS Magenta red
2. Best Carmine - Bright red
3. Langhan’s Iodine - Mahogany brown

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16
Q

ACID MUCOPOLYSACCARIDE STAINS

A
  1. Metachromatic stains
  2. Colloidal Iron technique
  3. Gomori’s aldehude Fuchsin stain
  4. Fluorescent acridine orange technique
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17
Q

Metachromatic stains

A
  1. Toluidine blue and Azure A
  2. Uranyl Nitrate
18
Q

FIBRIN STAINS

A
  1. PAS
  2. Gram’s stain
  3. MPTAH
  4. H & E
19
Q

AMYLOID STAINS

A
  1. Congo red- red/deep pink
  2. Gram’s iodine- blue
  3. Krajian’s- red
  4. Induced fluorescence
  5. Metachromatic
20
Q

MUSCLE STAINS

A
  1. MPTAH - black
    2.Lissamine fast red- red
21
Q

BONE STAINS

A
  1. Heidenhain’s s iron hematoxylin with eosin - red
    2.Van Gieson’s Picrofuchsin - bright red
    3.Masson’s trichrome - blue/green
22
Q

Nervous System Stains:

Stains neurons, Axons and neurofibrils

A

Bielchowsky’s

23
Q

Excellent stain for Astrocytes

A

Mallory’s Phosphotungstic acid Hematoxylin
(MPTAH)

24
Q

Nervous sytem stain for astrocytes

A

Cajal’s Gold Sublimate

25
Q

Silver impregnation technique stains

A

Neuroglia

26
Q

Nervous system stain for myelin sheath

A
  1. Osmic acid
  2. Weigert PAL
  3. Sudan black
27
Q

Nervous system stains for Nissl bodies/Tigroid substances

A
  1. H & E
  2. Thionine - purple
  3. Toluidine blue - deep blue
  4. Methylene blue - deep blue
  5. Cresyl fast violet
28
Q

Tissue Pigments and Deposit Stains:

For Hemoglobin (black)

A

Benzidine

29
Q

Tissue Pigments and Deposit Stains:

For Hemosiderin (deep blue)

A

Perl’s Prussian Blue

30
Q

Tissue Pigments and Deposit Stains:

For Ferrous ion (blue)

A

Turnbull’s Blue reaction

31
Q

Tissue Pigments and Deposit Stains:

For bile pigments (green)

A

Gmelin’s and stein’s Iodine test

32
Q

Tissue Pigments and Deposit Stains:

For Hemofuchsin (red)

A

Mallory’s Fuchsin

33
Q

Tissue Pigments and Deposit Stains:

For Melanin (black)

A

Masson Fontana

34
Q

General bacteria stain

A

Gram’s stain

35
Q

for AFB

A

Ziehl Neelsen’s

36
Q

stain for Leishmania, Malaria, trypanosomes, spirochetes and toxoplasmas

A

Rapid Giemsa

37
Q

for Spirochetes & leprae cells

A

Levaditi

38
Q

In Levaditi, Spirochetes stains _ on a _ background

A

BLACK : YELLOWISH BROWN

39
Q

In Warthin Starry, Spirochetes stains _ on a _ background

A

BLACK : GOLDEN YELLOW

40
Q

For Helicobacter pylori

A

Warthin Starry

Histopathology (5 decks)

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