COUNTERSTAINS Flashcards
RED cytoplasmic stains
Eosin Y
Eosin B
Phloxine B
Rose Bengal
YELLOW cytoplasmic stains
Picric acid
Orange G
GREEN cytoplasmic stains
Light green SF
Lissamine green
RED nuclear stain
Carmine
Neutral Red
Safranin
BLUE nuclear stain
Methylene blue
Toluidine blue
Celestine blue
Hematoxylin
simplest connective tissue stain
Van Gieson’s
uses acid stain; no color
Masson’s trichrome
can also stain hyaline fibrils, amyloid and smooth muscles
Mallory’s Aniline blue
for Renal Glomerular basement membraine staining
Azocarmine
ELASTIC FIBERS STAINS
- Weigert’s Elastic tissue stain (dark blue/blue black)
- Taenzer-Unna Orcein Method (dark brown)
- Verhoeff’s stain
- Masson’s trichrome
- Krajian’s (bright blue)
RETICULUM STAINS
- Silver impregnation technique
- PAS
- Bielschowsky
- Periodic acid leucofuchsin technique
- Gold method
Carbohydrate stain that produce magenta red color
PAS
most common method used for the demonstration of glomerular basement membrane of kidney
PAS Technique
PAS positive substances
- Carbohydrates
- Phopholipids
- Glycolipids
- Glycoproteins
- Unsaturated lipids
- Mucoproteins
(CAPGGUM)
GLYCOGEN STAINS
1.PAS Magenta red
2. Best Carmine - Bright red
3. Langhan’s Iodine - Mahogany brown
ACID MUCOPOLYSACCARIDE STAINS
- Metachromatic stains
- Colloidal Iron technique
- Gomori’s aldehude Fuchsin stain
- Fluorescent acridine orange technique
Metachromatic stains
- Toluidine blue and Azure A
- Uranyl Nitrate
FIBRIN STAINS
- PAS
- Gram’s stain
- MPTAH
- H & E
AMYLOID STAINS
- Congo red- red/deep pink
- Gram’s iodine- blue
- Krajian’s- red
- Induced fluorescence
- Metachromatic
MUSCLE STAINS
-
MPTAH - black
2.Lissamine fast red- red
BONE STAINS
-
Heidenhain’s s iron hematoxylin with eosin - red
2.Van Gieson’s Picrofuchsin - bright red
3.Masson’s trichrome - blue/green
Nervous System Stains:
Stains neurons, Axons and neurofibrils
Bielchowsky’s
Excellent stain for Astrocytes
Mallory’s Phosphotungstic acid Hematoxylin
(MPTAH)
Nervous sytem stain for astrocytes
Cajal’s Gold Sublimate
Silver impregnation technique stains
Neuroglia
Nervous system stain for myelin sheath
- Osmic acid
- Weigert PAL
- Sudan black
Nervous system stains for Nissl bodies/Tigroid substances
- H & E
- Thionine - purple
- Toluidine blue - deep blue
- Methylene blue - deep blue
- Cresyl fast violet
Tissue Pigments and Deposit Stains:
For Hemoglobin (black)
Benzidine
Tissue Pigments and Deposit Stains:
For Hemosiderin (deep blue)
Perl’s Prussian Blue
Tissue Pigments and Deposit Stains:
For Ferrous ion (blue)
Turnbull’s Blue reaction
Tissue Pigments and Deposit Stains:
For bile pigments (green)
Gmelin’s and stein’s Iodine test
Tissue Pigments and Deposit Stains:
For Hemofuchsin (red)
Mallory’s Fuchsin
Tissue Pigments and Deposit Stains:
For Melanin (black)
Masson Fontana
General bacteria stain
Gram’s stain
for AFB
Ziehl Neelsen’s
stain for Leishmania, Malaria, trypanosomes, spirochetes and toxoplasmas
Rapid Giemsa
for Spirochetes & leprae cells
Levaditi
In Levaditi, Spirochetes stains _ on a _ background
BLACK : YELLOWISH BROWN
In Warthin Starry, Spirochetes stains _ on a _ background
BLACK : GOLDEN YELLOW
For Helicobacter pylori
Warthin Starry
