Other Stains Used

Question 1

mixture of picric acid and acid fucshin for the demonstration of connective tissues, mucin and elastic fibers

Answer 1

Van Gieson’s

Question 2

a basic acridine fluorochrome which permits the discrimination between dead and living cells

Answer 2

Acridine Orange

Question 3

Acridine orange gives ____ fluorescence for DNA

Answer 3

Green

Question 4

Acridine orange gives ____ fluorescence for RNA

Answer 4

Red

Question 5

for calcium salts and phosphatase activity

Answer 5

Acridine Red 3B

Question 6

stain acid mucopolysaccharides specifically connective tissue and epithelial mucin

Answer 6

Alcian Blue

Question 7

A cytoplasmic stain used for counterstaining of epithelial sections

Answer 7

Aniline blue

Question 8

A plasma stain utilized also for deep staining of acid fast organisms

Answer 8

Basic Fuchsin

Question 9

For staining hemoglobin

Answer 9

Benzidine

Question 10

For staining diphtheria

Answer 10

Bismarck brown

Question 11

Used as a contrast stain for Gram’s technique, acid fast and Papanicolaou method

Answer 11

Bismarck brown

Question 12

used as a chromatin stain for fresh material in smear prep

Answer 12

Carmine

Question 13

Comibined with aluminum chloride to stain glycogen

Answer 13

Best carmine

Question 14

A mordanted dye acting as a basic dye and staining acid substances

Answer 14

Mayer’s Carmalum solution

Question 15

Recommended for routine taining of fixed sections

Answer 15

Celestine blue

Question 16

Best known as indicator; may be utilized as a stain for axis cylinders in embryos

Answer 16

Congo Red

Question 17

used for staining elastic tissues, amyloid and myelin (Krajian’s method)

Answer 17

Congo Red

Question 18

A nuclear chromatin stain used for staining amyloid in frozen sections and platelets

Answer 18

Crystal Violet

Question 19

Mixture of crystal violet, methyl violet and Dextrin

Answer 19

Gentian Violet

Question 20

Used for staining blood to differentiate leukocytes

Answer 20

Giemsa

Question 21

Used for metallic impregnation

Answer 21

Gold sublimate

Question 22

Gold sublimate is made up of

Answer 22

Gold chloride and Mercuric chloride

Question 23

The oldest of all stain

Answer 23

Iodine

Question 24

Stains amyloid, cellulose, starch, carotenes and glycogen

Answer 24

Iodine

Question 25

An Intravital stain used for demonstrating mitochondria

Answer 25

Janus Green B

Question 26

Used as a contrast for staining Ascaris eggs and erythrocytes

Answer 26

Malachite Green

Question 27

Used as bacterial spore stain

Answer 27

Malachite Green

Question 28

Stains chromatin green in the presence of an acid

Answer 28

Methyl Green

Question 29

Stain for plasma cells, fresh sputum for malignant cells

Answer 29

Methylene Blue

Question 30

Evaluation and differentiation of bacterial organisms. Diptheria diagnosis and Nervous tissue vital staining

Answer 30

Methylene Blue

Question 31

Used in frozen sections for rapid diagnosis

Answer 31

Loeffler's Polychrome

Question 32

Coloring nuclei of leukocytes reddish purple in the presence of methylene blue

Answer 32

Methylene Violet

Question 33

For observing cell granules and vacuoles of phagocytic cell

Answer 33

Neutral Red

Question 34

Substitute for carbol fuchsin in acid fast staining

Answer 34

Night Blue

Question 35

Excellent stain for Elastic fibers

Answer 35

Orcein

Question 36

Demonstrates the finest and most delicate fibers in skin

Answer 36

Orcein

Question 37

Stain used in Dermatologic studies

Answer 37

Orcein

Question 38

Used as a fixative

Answer 38

Osmium tetroxide

Question 39

Used to stain fats

Answer 39

Osmium tetroxide

Question 40

Used a microanatomical contrast stain

Answer 40

Prussian Blue

Question 41

Used with osmic acid to fix and stain blood and glandular tissues

Answer 41

Rhodamine B

Question 42

For demonstration for circulatory system by injecting

Answer 42

Prussian Blue

Question 43

Used in identification of Spirochetes

Answer 43

Silver Nitrate

Question 43

Recommended for staining of Nissl granules or chromophilic bodies

Answer 43

Toluidine Blue

Question 44

Nuclear stain for fixed tissues

Answer 44

Toluidine Blue

Question 45

Used as a substitute for thionine in fresh tissue sections

Answer 45

Toluidine Blue

Question 46

For demonstration of neuroglia in frozen sections

Answer 46

Victoria Blue

Question 47

Greatest affinity for phospholipids or neutral fats

Answer 47

Sudan Black B

Question 48

should be discarded if the brownish black color appeared

Answer 48

Sudan Black B

Question 49

Demonstrates lipids that are resistant to paraffin embedding

Answer 49

Sudan Black B

Question 50

Recommended for neutral fats (triglycerides)

Answer 50

Sudan IV / Sharlach R

Question 51

First sudan dye introduced in histochemistry

Answer 51

Sudan III

Question 52

NOT A LYSOCHROME

Answer 52

Oil Red O Osmic acid Nile Blue sulfate

Question 53

A good stain for the CNS

Answer 53

Sudan III

Question 54

METACHROMATIC DYES

Answer 54

* Basic fuchsin * Bismarck brown * Safranin * Methylene blue * Methyl Violet * Thionine * Toluidine blue * Azure A, B , C * Cresyl blue (BBSMMTT ACo)

Question 55

SUPRAVITAL STAINS

Answer 55

* Janus Green B * Nile Blue * Neutral Red * Thionine * Toluidine blue * Trypan blue (J-NN-TTT)