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Histopathology > Other Stains Used > Flashcards

Other Stains Used Flashcards

1
Q

mixture of picric acid and acid fucshin for the demonstration of connective tissues, mucin and elastic fibers

A

Van Gieson’s

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2
Q

a basic acridine fluorochrome which permits the discrimination between dead and living cells

A

Acridine Orange

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3
Q

Acridine orange gives ____ fluorescence for DNA

A

Green

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4
Q

Acridine orange gives ____ fluorescence for RNA

A

Red

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5
Q

for calcium salts and phosphatase activity

A

Acridine Red 3B

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6
Q

stain acid mucopolysaccharides specifically connective tissue and epithelial mucin

A

Alcian Blue

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7
Q

A cytoplasmic stain used for counterstaining of epithelial sections

A

Aniline blue

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8
Q

A plasma stain utilized also for deep staining of acid fast organisms

A

Basic Fuchsin

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9
Q

For staining hemoglobin

A

Benzidine

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10
Q

For staining diphtheria

A

Bismarck brown

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11
Q

Used as a contrast stain for Gram’s technique, acid fast and Papanicolaou method

A

Bismarck brown

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12
Q

used as a chromatin stain for fresh material in smear prep

A

Carmine

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13
Q

Comibined with aluminum chloride to stain glycogen

A

Best carmine

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14
Q

A mordanted dye acting as a basic dye and staining acid substances

A

Mayer’s Carmalum solution

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15
Q

Recommended for routine taining of fixed sections

A

Celestine blue

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16
Q

Best known as indicator; may be utilized as a stain for axis cylinders in embryos

A

Congo Red

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17
Q

used for staining elastic tissues, amyloid and myelin (Krajian’s method)

A

Congo Red

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18
Q

A nuclear chromatin stain used for staining amyloid in frozen sections and platelets

A

Crystal Violet

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19
Q

Mixture of crystal violet, methyl violet and Dextrin

A

Gentian Violet

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20
Q

Used for staining blood to differentiate leukocytes

A

Giemsa

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21
Q

Used for metallic impregnation

A

Gold sublimate

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22
Q

Gold sublimate is made up of

A

Gold chloride and Mercuric chloride

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23
Q

The oldest of all stain

24
Q

Stains amyloid, cellulose, starch, carotenes and glycogen

25
Q

An Intravital stain used for demonstrating mitochondria

A

Janus Green B

26
Q

Used as a contrast for staining Ascaris eggs and erythrocytes

A

Malachite Green

27
Q

Used as bacterial spore stain

A

Malachite Green

28
Q

Stains chromatin green in the presence of an acid

A

Methyl Green

29
Q

Stain for plasma cells, fresh sputum for malignant cells

A

Methylene Blue

30
Q

Evaluation and differentiation of bacterial organisms. Diptheria diagnosis and Nervous tissue vital staining

A

Methylene Blue

31
Q

Used in frozen sections for rapid diagnosis

A

Loeffler’s Polychrome

32
Q

Coloring nuclei of leukocytes reddish purple in the presence of methylene blue

A

Methylene Violet

33
Q

For observing cell granules and vacuoles of phagocytic cell

A

Neutral Red

34
Q

Substitute for carbol fuchsin in acid fast staining

A

Night Blue

35
Q

Excellent stain for Elastic fibers

36
Q

Demonstrates the finest and most delicate fibers in skin

37
Q

Stain used in Dermatologic studies

38
Q

Used as a fixative

A

Osmium tetroxide

39
Q

Used to stain fats

A

Osmium tetroxide

40
Q

Used a microanatomical contrast stain

A

Prussian Blue

41
Q

Used with osmic acid to fix and stain blood and glandular tissues

A

Rhodamine B

42
Q

For demonstration for circulatory system by injecting

A

Prussian Blue

43
Q

Used in identification of Spirochetes

A

Silver Nitrate

43
Q

Recommended for staining of Nissl granules or chromophilic bodies

A

Toluidine Blue

44
Q

Nuclear stain for fixed tissues

A

Toluidine Blue

45
Q

Used as a substitute for thionine in fresh tissue sections

A

Toluidine Blue

46
Q

For demonstration of neuroglia in frozen sections

A

Victoria Blue

47
Q

Greatest affinity for phospholipids or neutral fats

A

Sudan Black B

48
Q

should be discarded if the brownish black color appeared

A

Sudan Black B

49
Q

Demonstrates lipids that are resistant to paraffin embedding

A

Sudan Black B

50
Q

Recommended for neutral fats (triglycerides)

A

Sudan IV / Sharlach R

51
Q

First sudan dye introduced in histochemistry

52
Q

NOT A LYSOCHROME

A

Oil Red O
Osmic acid
Nile Blue sulfate

53
Q

A good stain for the CNS

54
Q

METACHROMATIC DYES

A
  • Basic fuchsin
  • Bismarck brown
  • Safranin
  • Methylene blue
  • Methyl Violet
  • Thionine
  • Toluidine blue
  • Azure A, B , C
  • Cresyl blue

(BBSMMTT ACo)

55
Q

SUPRAVITAL STAINS

A
  • Janus Green B
  • Nile Blue
  • Neutral Red
  • Thionine
  • Toluidine blue
  • Trypan blue

(J-NN-TTT)

Histopathology (5 decks)

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