ADHESIVES Flashcards
used in order to promote adhesion of sections to slides
Adhesives
Adhesives are spread thinly and evenly on a _______
clean grease-free glass slide
most commonly used because it is very easy to make and relatively inexpensive
Mayer’s Egg Albumin
Formula for Mayer’s Egg Albumin
- Egg white (50mL)
- Glycerin (50mL)
- Thymol crystals (100mg)
prevents mold formation
Thymol crystals
(thyMOLD crystals 😭)
Formula for Dried Albumin
- Dried Albumin (5g)
- Sodium chloride (5g)
- Distilled water (100mL)
- Thymol crystals
Formula for 1% Gelatin
- Gelatin (1g)
- Distilled water (100mL)
- Glycerol (15mL)
- Phenol crystals (2g)
prevents mold formation in 1% Gelatin
Phenol crystals
Formula for Strach Paste
- Powdered starch (1g)
- Distilled watter (30mL = 10mL cold; 20 mL boiling)
- Hyrdochloric acid (2 drops)
- Thymol crystals
readily avaiable from outdated blood stored in blood banks
Plasma
0.1% aqueous detergent solution which is further diluted 1:10 with distilled water prior use
Poly-L-Lysine
Used as a section adhesive in immunohistochemistry
Poly-L-Lysine
very useful in cytology particularly for cytospin of proteinaceous or bloody materials
3-APES
Best adhesive agent
3-APES