Stains Flashcards
aqueous or alcohol solution of a single basic dye
Simple Stain
determines cellular shapes & arrangements
Simple Stain
Methylene Blue
Simple Stain
Malachite Green
Simple Stain
Gram Stain: Primary Stain
Crystal Violet
Gram Stain: Mordant
Iodine
Gram Stain: Neutralizer
Acid alcohol
Gram Stain: Secondary Stain
Safranin
Acid Fast: Hot Method
Ziehl-Nielssen Method
Acid Fast: Cold Method
Kinyoun
Acid Fast: Hot Method
Primary Stain
Carbol-Fuschin (Red)
Acid Fast: Hot Method
Mordant
Heat
Acid Fast: Hot Method
Decolorizer
3% Acid Alcohol
Acid Fast: Hot Method
Counterstain
Methylene Blue
Acid Fast: Hot Method
Positive Result
Red
Acid Fast: Hot Method
Negative Result
Blue
Acid Fast: Cold Method
Primary Stain
Carbol-Fuschin (Red)
Acid Fast: Cold Method
Mordant
Phenol
Acid Fast: Cold Method
Decolorizer
3% Acid Alcohol
Acid Fast: Cold Method
Counterstain
Malachite Green
Acid Fast: Cold Method
Positive Result
Red
Acid Fast: Cold Method
Negative Result
Green
used to disctinguish Mycobacterium & Nocardia
Acid Fast Stain
Special Stains: Nigrosin/India Ink
Capsule
Special Stains: Schaeffer Fulton/ Writz and Conklin Method
Endospore
Special Stains: Welch Method
Capsule
Special Stains: Fuelgen Stain
Nucleic Acid
Special Stains: Leifson
Flagella
Special Stains: LAMB (Loeffler’s alkaline methylene blue)
Metachromatic Granule
Special Stains: For Spirochetes
(Get Well Soon)
Giemsa Stain
Silver Stain
Wright Stain
Special Stains: Negative
Nigrosin
India Ink
Congo Red