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MOD 6 - Microbiology and Parasitology > Culture Medium > Flashcards

Culture Medium Flashcards

1
Q

Medium for M.tuberculosis

A

Lowenstein Medium

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2
Q

Medium for Neisseria gonorrhea and Neisseria meningitidis

A

Modified Thayer Martin Agar

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3
Q

Medium for gram negative

A

MacConkey Agar

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4
Q

Medium Leptospira interrogans

A

Fletcher’s Media

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5
Q

Medium for Haemophilus influenzae

A

Chocolate Agar

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6
Q

Classify according to CONSISTENCY

Brain Heart Infusion (BHI)

A

Liquid/Nutrient Broth

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7
Q

Classify according to CONSISTENCY

Tryptone Soya Bean/ Soy Bean Casein Digest

A

Liquid/Nutrient Broth

For fungi and anaerobes

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8
Q

Classify according to CONSISTENCY

Thioglycollate

A

Liquid/nutrient broth

Mainly anaerobic, sometimes w microaero and aerobic

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9
Q

Classify according to CONSISTENCY

contains no agar

A

Liquid/ nutrient broth

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10
Q

Classify according to CONSISTENCY

contains 0.5-1%

A

Semi-solid medium

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11
Q

Classify according to CONSISTENCY

Sulfide indole motility (SIM)

A

Semi-solid

Differentiate gram (-) bacteria

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12
Q

Classify according to CONSISTENCY

contains 2-3%

A

Solid

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13
Q

Classify according to CONSISTENCY

Triple sugar iron (TSI)

A

Solid

Differentiate enterics based on the ability to reduce sulfur

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14
Q

Classify according to CONSISTENCY

Mac Conkey Agar

A

Solid

Gram (-), differentiate w/ gram +

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15
Q

Classify according to CONSISTENCY

Chocolate Agar

A

Solid

H. influenzae

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16
Q

Classify according to COMPOSITION

all substances are known
for research purposes

A

Synthetic/Defined

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17
Q

Classify according to COMPOSITION

some substances are unknown
for isolation of bacteria

A

Non-Synthetic/ complex medium

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18
Q

Classify according to COMPOSITION

Nutrient broth

A

Non-Synthetic/ complex medium

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19
Q

Classify according to COMPOSITION

Mac Conkey Agar

A

Non-Synthetic/ complex medium

20
Q

Classify according to COMPOSITION

Tryptone Soya Bean/Soya Bean Cassein Digest

A

Non-Synthetic/ complex medium

21
Q

Classify according to COMPOSITION

isolate obligate intracellular

A

Tissue culture medium

22
Q

Classify according to COMPOSITION

W138

A

Tissue culture medium

human cell kidney culture line

23
Q

Classify according to COMPOSITION

HELA 229

A

Tissue culture medium

chlamydia

24
Q

Classify according to COMPOSITION

Mc Coy Cells

A

Tissue culture medium

chlamydia

25
Q

Classify according to DISTRIBUTION

butt, butt-slant, slant, liquid media

A

Tubed media

26
Q

Classify according to DISTRIBUTION

petri dish

A

Plated media

27
Q

Classify according to USE

routinely used in laboratories
w/out added supplements

A

Simple/general purpose

28
Q

Classify according to USE

nutrient agar

A

Simple/general purpose

29
Q

Classify according to USE

nutrient broth

A

Simple/general purpose

30
Q

Classify according to USE

tryptone soya bean/ soy bean cassein digest

A

Simple/general purpose

31
Q

Classify according to USE

contain specific nutrients

A

Enrichment medium

32
Q

Classify according to USE

Alkaline peptone water

A

Enrichment media

Vibrio

33
Q

Classify according to USE

Selenite F

A

Enrichment media

Salmonella

34
Q

Classify according to USE

Buffered charcoal yeast extract

A

Enrichment media

legionella, pneumophila

35
Q

Classify according to USE

Thioglycollate

A

Enrichment media

Mainly anaerobic, sometimes w microaero and aerobic

36
Q

Classify according to USE

w/ added supplement for fastidious organisms

A

Enriched media

37
Q

Classify according to USE

Blood agar plate

A

Enriched media

nutrient agar + 5% sheep red blood cell

38
Q

Classify according to USE

Chocolate agar

A

Enriched media

nutrient agar + powdered Hgb

39
Q

Classify according to USE

allows visualization of metabolic differences between species

A

Differential media

40
Q

Classify according to USE

Mac Conkey

A

Differential media
selective

differentiates G- from G+
gram - lactose fermenter = pink

41
Q

Classify according to USE

Blood agar plate (BAP)

A

Differential media

hemolytic bacteria

42
Q

Classify according to USE

Eosin methylene blue

A

Differential media

G+ and G-

43
Q

Classify according to USE

hektoen enteric agar

A

Differential media
selective media

differentiate salmonella & shigella from other entero

44
Q

Classify according to USE

promote growth of desire organism

A

Selective media

45
Q

Classify according to USE

xylose lysine decarboxylase

A

Selective media

shigella & salmonella

46
Q

Classify according to USE

bisulfide agar

A

Selective media

salmonella

47
Q

Classify according to USE

mannitol salt agar (7.5%)

A

Selective media

for s.aureus

mannitol fermenting (lowerarea) - yellow
non-fermenting (upper area)

MOD 6 - Microbiology and Parasitology (13 decks)

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