Bacteriology - Dx and Tests

Question 1

Streptococcus pneumoniae

Answer 1

quellung - methylene blue (capsule)
optochin test - sensitive (VROPS)

Question 2

Streptococcus viridans

Answer 2

optochin - resistant (VROPS)

Question 3

Bacillus anthracis

Answer 3

M’Fadyean reaction

Question 4

Clostridium botulinum (botox)

Answer 4

Mouse neutralization test

Question 5

Clostridium perfringens (letcithinase)

Answer 5

Nagler’s reaction

Question 6

Corynebacterium diphtheriae (tests)

Answer 6

Loeffler’s coagulated serum medium
Tellurite containing differential medium

Question 7

Corynebacterium diphtheriae (dx)

Answer 7

Elek test - in vitro
Schick - in vivo
Loeffler’s slant (laboratory)

Question 8

Salmonella typhi

Answer 8

widal reaction - antigen
typhidot - IgM & IgG

Question 9

Helicobacter pylori

Answer 9

C13 & C14 (radioactive)
urea breath test

Question 10

Bordetella pertussis

Answer 10

Regan-lowe or bordet-gangou medium

Question 11

Neisseria

Answer 11

Thayer-Martin agar (VCN)
Modified (VCN + sulfamethoxazole)

vancomycin (-) g+
collistin (-) g- except neisseria
nystatin (-) fungi
sulfamethoxazole (-) g+

Question 12

Mycobacterium tuberculosis

Answer 12

Purified protein derivative (PPD) / mantoux test/ tuberculin skin test

Question 13

Mycobacterium leprae

Answer 13

Lepromin skin test

Question 14

Campylobacter jejuni

Answer 14

Campy or skirrow’s agar

Question 15

Yersinia pestis

Answer 15

wayson’s stain

Question 16

Borrelia burgdorferi

Answer 16

giemsa stain

Question 17

francisella tularensis

Answer 17

immunofluorescence stain