Staining

Question 1

Name 4 staining techniques

Answer 1

• Gram staining
• Acid fasting
• Wright’s stain
• Negative staining

Question 2

Explain what occurs in gram staining

Answer 2

• Used to differentiate between bacteria
• Iodine used to identify gram positive bacteria
• Safranin she used to identify gram negative bacteria

Question 3

Explain how and why acid fast is used

Answer 3

• differentiates between mycobacteria and bacteria
• used of carbonfuchin dye
• mycobacteria will retain stain

Question 4

Explain Wright’s stain

Answer 4

• differentiates between blood cell types
• mixture of eosin and methylene blue dye
• red blood cells look yellowish red
• neutrophils has a dark purple nuclei

Question 5

Go over the steps of slide production

Answer 5

•Fixing
Chemicals added to perverse effect the specimen
•sectioning
Specimen dehydrated, hardened with resin and cut into sections called microtome
•staining
Stains are added
•mounting
The specimen is secured into place with a cover slip over it