Microscopy

Question 1

Definition of magnification

Answer 1

The number of times an image is larger then its real size

Question 2

Definition of resolution

Answer 2

The ability to be able to distinguish 2 separate points

Question 3

What are the 3 types of microscopes used

Answer 3

• Light microscope
• electron microscope
  
  • scanning microscope
  • transmission microscope

Question 4

Facts about light microscope and pros and cons

Answer 4

•use of eye piece 
•use of light for illumination
•resolution of 0.2 micrometers 
\+cheap
\+specimen can be alive
\+simple prep
-low resolution
-x1500 zoom
-specimen is sliced thin

Question 5

Facts about and pros and cons of transmission microscope

Answer 5

•use of electrons that will pass through sample
•specimen must be dead
•x500,000 zoom
•resolution of 0.5 nanometers
\+large magnification
\+clear resolution
-expensive
-specimen must be dead
-specimen prep need expertise

Question 6

Facts about scanning microscope, pros and cons

Answer 6

•uses electrons to bounce off specimen 
•electrons used for focusing 
•specimen must be dead
•x100,000 zoom
•resolution of 10 nanometers 
\+3D image given
\+clear resolution 
\+large magnification
-expensive
-needs vacuum 
-prep of specimen

Question 7

What is an artefacts

Answer 7

Features shown in a specimen sample due to an error in the prep process