Sporozoa Plasmodium Flashcards
Can cause malaria
Plasmodium spp.
What are the Plasmodium spp.?
P. falciparum
P. vivax
P. ovale
P. malariae
Lab diagnosis of Plasmodium spp.
Peripheral blood:
- Giemsa stain
- Wright’s stain
What P. spp can be mixed infections? (Most frequent)
P. vivax and P. falciparum
When is the best time of blood collection for diagnosis of P. spp?
During episodes of Paroxysms
at what hour does Paroxysms happen?
Every 6-12hrs up to 48hrs
2 qualitative analysis for P. spp
Thick smear
Thin smear
Dehomogenized smear to know if positive or negative for Plasmodium spp
Thick smear
Smear for specification of exact specie of P. spp
Thin smear
Usual start of the life cycle of Plasmodium spp. By taking?
Mosquitoes taling blood meal and inject the sporozoite
Stage occuring in liver
Exoerythrocytic stage
What the sporozoite does in exoerythrotic stage
Penetrates liver cells,
becomes a Schizont,
Rupture of liver cells w/ the schizont and RELEASE merozoites
Upon the release of Merozoites, what can happen to the infected human?
Chills and fever
Merozoites can also cause episodes of?
Paroxysms
Stage after exoerythrocytic stage
Erythrocytic stage
What happens in erythrocytic stage
Schizonts release sporozoite to infect other RBC
4 morphologic form of p
Plasmodium spp. In humans:
Ring form (immature troph)
Trophozoite
Schizont
Gametocyte
Serologic tests for Plasmodium spp.
Malarial Rapid Diagnostic Test
Immunochromatographic method
Antigens used in serologic test
Histidine Rich Protein II (HRP II)
Plasmodium Lactate Dehydrogenase (PLDH)
Antigen for P. falciparum
Histidine Rich Protein II
Benign tertian malaria
Plasmodium vivax
Plasmodium vivax appearance of mature
Ameboid
Plasmodium vivax
Any morphologic form can be seen
Plasmodium ovale
All morphologic form can be seen
Ring form of P. ovale
Larger than vivax
Trophozoite of ovale
No notable ameboid appearance
Schizont of ovale
Rosette formation
-the choice to identify ovale from vivax
Gametocyte of ovale
Smaller than vivax
Quadnucleated
Plasmodium malaria
Ring form of P. malaria
Smaller than viva
Rarelly visible in routine
Usually cultured
Trophozoite of P. malaria
Cytoplasm is solid
Darker than vivax and ovale
In matured: no spaces in cytoplasm
Schizonts of P. malaria
Irregular rosette arragement
Basket appearance
Ring form of P. falciparum
Chromatin dot and cytoplasmic ring are smaller than vivax
-usually cannot be found
HEADPHONE CONFIGURATION
Developing troph of falciparum
Heavy rings
Schizont of falciparum
Mas kalat, RBCs cannot be seen
Gametocyte of falciparum
Sausage shape or cresent or banana shape
Plasmodium knowlesi
Commonly found in monkeys
Maurer’s dot
Plasmodium falciparum
Schuffner’s dot
Plasmodium vivax
James dot
Plasmodium ovale
Ziemann’s dot
Plasmodium