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MLT Microbiology > Specimen Processing > Flashcards

Specimen Processing Flashcards

1
Q

Media for Throat and Nasopharyngeal (6 and under)

A

BAP, 35 C aerobic
CHOC, 35 C in CO2

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2
Q

Normal Flora for throat and nasopharyngeal (6 and under)

A
  • alpha and gamma strep
  • Commensalism Neisseria
  • S. Epidermiditis diptheroids
  • S. Pneumoniae Anaerobes
  • N Meningitidis
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3
Q

Common pathogens in throat

A
  • Group A- beta hemolytic strep
  • H. Influenza
  • C. Diphtheria
  • B. Pertussis
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4
Q

Common pathogens in nasopharyngeal (6 and under)

A

Group A-beta hemolytic strep
N. Meningitides
S. Pneumoniae
H. Influenza

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5
Q

What do all negative strep screens need?

A

Throat culture

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6
Q

Media for Respiratory

A
  • CHOC, 35C n CO2
  • MacConkey, aerobic
  • Anaerobic media
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7
Q

Normal Flora for Respiratory

A
  • Alpha and gamma strep
  • commensal Neisseria
  • S. Epidermidis diptheroids
  • Haemophilus
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8
Q

Common pathogens of respiratory

A
  • Group A - beta hemolytic strep
  • H. Influenza
  • S. Aureus
  • Pseudomonas species
  • S. Pneumoniae
  • Enterobacteriaceae
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9
Q

Describe the loop used for urine cultures

A

Platinum loop: 0.001 or 0.01

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10
Q

Media for Urine (clean catch)

A

MacConkeys, 35C aerobic

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11
Q

Normal flora for urine

A

None

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12
Q

Common pathogens of urine

A
  • E. Coli and other enterics
  • Enterococci
  • Pseudomonas Species
  • Staphylococci aureus
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13
Q

Media for stool

A
  • BAP
  • MacConkeys
  • EMB
  • XLD
  • HE
  • SS
  • Selenite F, 35 C aerobic
  • Campylobacter plate or Skirrows in 45C, 5% in O2
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14
Q

Media for bloody stool

A

Mac with sorbitol

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15
Q

Normal flora for stool

A
  • Anaerobes
  • enterobacteriaceae
  • Enterococci
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16
Q

Common pathogens in stool

A
  • Salmonella species
  • Shigella species
  • E. Coli
  • Clostridium difficile ( if bloody and patient on antibiotics)
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17
Q

What should be commented on in stool culture?

A

Staph or yeast

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18
Q

Media for genital

A

Modified Thayer-Martin, 35C in CO2

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19
Q

Normal flora for genital

A
  • Lactobacilli
  • Corynebacterium
  • CN Staph species
20
Q

Common pathogens for genital

A

N. Gonorrhoeae

21
Q

What stain is used for genital?

A

Gram stain

22
Q

What is considered sterile fluids?

A
  • CSF
  • Pleura
  • Joint
  • peritoneal
  • etc..
23
Q

Media for sterile fluids

A
  • blood agar
  • CHOC agar, 35C in CO2
  • Thioglycolate
  • Send out anaerobes in chopped meat or thio
  • for peritoneal, set up Mac
24
Q

Normal Flora for sterile fluids

25
Q

Common pathogens of sterile fluids

A

Any isolate potentially significant

26
Q

Stain used for sterile fluids

A
  • gram stain
  • CSF = India ink and hold 72-96 hours)
27
Q

Media for blood

A
  • submitted in 2 bottles: one 35C aerobic and one 35C anaerobic
28
Q

Media for positive blood culture

A
  • plate to: BAP, 35 C
  • CHOC agar, 35C in CO2
29
Q

Normal flora for blood

30
Q

Common pathogens in blood

31
Q

Common pathogens in positive blood culture

A

Any isolate potentially significant

32
Q

What stain is used for positive blood cultures?

A

Gram stain

33
Q

Media for vaginal

A
  • BAP
  • CHOC, 35C, CO2
  • MacConkeys
  • thioglycolate
  • potato dextrose agar
  • Columbia Colistin
  • Nalidixic acid agar (CAN)
  • Vaginitis agar
  • Modified Thayer-Martin 35C, CO2
34
Q

Normal flora for vaginal

A
  • Lactobacilli
  • Acinetobacter
  • Corynebacterium
  • CN staph
  • Doderlein bacilli
35
Q

Describe Doderlein Bacilli

A

Mixture containing yeasts, corynebacteria, and mycoplasmas

36
Q

Common pathogens in vaginal

A
  • N. gonorrhoeae
  • Gardenerella vaginalis
  • Candida albicans
  • S. Aureus ( if predominant)
  • ## Bacteroides species (anaerobe - if predominant)
37
Q

What stain is used for vaginal

A

Gram stain

38
Q

Describe listeria of vaginal

A

Listeria looks like beta-strep but is catalase positive

39
Q

Media for superficial wounds (ears and eyes included)

A
  • BAP
  • CHOC 35C in CO2
  • MAC
  • PEA
  • 2 anaerobic plates 35C
  • anaerobic
40
Q

Normal flora in superficial wound

A
  • bacillus species
  • S. Epidermidis diptheroids
  • S. Aureus
  • Micrococci
  • Peptococcus
  • Propionebacterium Acne’s
  • Candida albicans
  • Commensal Neisseria
  • Mycobacterium
  • Clostridium
  • Erysipelothrix rh
  • many more anaerobes
41
Q

Common pathogens of superficial wound

A
  • S. Aureus
  • Beta-hemolytic strep
  • P. Aeruginosa
  • H. Aegypticus (eye)
  • H. Influenza (biotype III)
  • Enterobacteriaceae
42
Q

Stain used for wounds

A

Gram stain

43
Q

Media for deep wound

A
  • same as superficial wound except thioglycolate broth or schadlers Broth
44
Q

Normal flora for deep wounds

45
Q

Common pathogens of deep wounds

A
  • S. Aureus
  • Beta-hemolytic strep
  • P. Aeruginosa
  • H. Aegypticus (eye)
  • H. Influenza (biotype III)
  • Enterobacteriaceae
  • anaerobes
  • isolates
  • identify any growth

MLT Microbiology (9 decks)

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