Special culture requirements

Question 1

H. Influenzae

Answer 1

Chocolate agar with factors V (NAD+) and X (hematin)

Question 2

N. gonorrhoeae, N. Meningitidis

Answer 2

Thayer Martin or VPN media, Vancomycin(inhibits gram positive) Polymyxin (inhibits gram negative organisms except neisseria,) and Nystatin(inhibits fungi)

Question 3

B. Pertussis

Answer 3

Bordet-Gengou patato agar

Question 4

C. Diptheriae

Answer 4

Tellurite Agar, Loffler medium

Question 5

M. Tuberculosis

Answer 5

Lowenstein Jensen agar

Question 6

M. Pneuomoniae

Answer 6

Eaton agar, requires Cholesterol

Question 7

Lactose fermenting enterics

Answer 7

pink colonies on macConkey agar,(acid turns pink)

Question 8

E. Coli is also grown on

Answer 8

Eosin Methylene Blue(EMB) agar (green metallic sheen)

Question 9

legionella

Answer 9

charoal yeast extract agar buffered with cysteine and iron

Question 10

Fungi

Answer 10

SABourad agar (SAB is a Fun Guy)