Sollars - DNA Structure Flashcards

1
Q

What is the central dogma?

A

DNA - Transcription - RNA - Translation - Protein

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2
Q

What is the general structure of DNA?

A

Polynucleotide sequence joined through 3’-5’ phosphodiester bonds. Polar primary structure, strands run antiparallel, exterior is hydrophilic, interior hydrophobic, helix has major and minor grooves

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3
Q

What is often found at the 5’ and 3’ ends of DNA?

A

5’ - Phosphate 3’ - Hydroxyl

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4
Q

What is the role of Actomycin D?

A

Anticancer drug which intercalates b/t the planar bases of DNA

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5
Q

What are Chargaff’s Rules?

A

A=T, G=C, Purines = Pyrimidines (A+G=T+C)

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6
Q

What is a major exception to Chargaff’s Rules?

A

Base composition in an organism can be affected by environment, age, and/or nutrition

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7
Q

What are the hydrogen bonding rules in DNA? What does this result in?

A

A-T = 2 G-C = 3 Sequences rich in A-T pairings will be weaker than those rich in G-C pairings

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8
Q

What is the most common form of DNA? What is Z-DNA? A-DNA?

A

B-DNA

Z-DNA: Left handed double helix rich in G-C

A-DNA: Made by dehydrating B-DNA, found in RNA hybrids or RNA-RNA double strands

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9
Q

What has an impact on viscosity and UV-absorption? What is this called, and how is it used?

A

Denaturation Hyperchromic Effect, used as standard to measure purity of DNA in preparations

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10
Q

Is melting point uniform for strands of DNA?

A

No, elevation/depression depends on differences in A/T , G/C rich regions (2 vs 3 hydrogen bonds)

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11
Q

What is Chromatin?

A

Complex of nucleic acids (DNA/RNA) and proteins (histones/nonhistones) comprising eukaryotic chromosomes

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12
Q

What is the role of histones in DNA secondary structure?

What is their electrochemical makeup?

What is the role of Histone H1?

A

DNA wraps around histone complexes to form nucleosomes

Large amounts of basic amino acids (H, K, R) leading to a positive charge which attracts negatively charged DNA

Histone H1 associated with linker DNA b/t nucleosomes, induces positive supercoiling in the linker region

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13
Q

What are the two types of Chromatin?

A

Euchromatin - loosely packed, transcriptionally active

Heterochromatin - tightly packed, transcriptionally inactive

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14
Q

What occurs when the axis of the DNA helix is coiled on itself?

What types of this are there?

What must cells do as a result?

A

Supercoiling

Positive - Winds in same direction as helix

Negative - Winds in opposite direction as helix

Cells must actively maintain an unwound state necessary to facilitate access to DNA

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15
Q

What class of molecules relax supercoiling stress?

What type of supercoiling to they maintain? How?

A

Topoisomerases

Maintain Negative Supercoiling by breaking DNA then resealing the breaks

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16
Q

What does Ciprofloxacin target?

A

Bacteria topoisomerase

17
Q

What does doxorubicin target?

A

Human type II topoisomerase inhibitor

18
Q

What drives the differences in packaging in viruses, bacteria, and higher organisms?

A

Genome size.

In humans, DNA is organized in chromosomes; in mitochondira and backteria they are circular (which are also loosely termed chromosomes)

19
Q

Centromere

What is different about metaphase?

A

Region to which spindle traction fibers attach during mitosis/meiosis.

Joining point for two replicated chromatids

During metaphase, centromere is narrower than distal regions, termed primary chromosomal constriction

20
Q

Telomere

A

Repeated DN sequence w/specialized proteins found and ends of eukaryotic chromosomes; chromosomes lose 100 nucleotides from ends per cell division (can be repaired by some cells)

21
Q

Origin of Replication

A

Nucleotide sequence at which DNA syntheis begins, multiple sites in humans

Bacteria only have one due to circular chromosome

22
Q

Rett Syndrome

A

X-linked genetic disorder observed in females; 99% of cases are mutations–lack of reproductive function.

Cause: Defect in methyl-CpG-binding protein 2, involved in chromatin formation—DNA packaging error

23
Q

What is Royal Jelly an example of?

A

Epigenetics–also a deviation from Chargaff’s rules

24
Q

DNA Methylation

Where does it usually occur?

What is the usual result?

A

Acts to sterically inhibit interaction with DNA molecules and further serve as a binding site for proteins

Usually occurs in the promoter region

Usually silences genes–but may also activate

25
Q

DNA Methyltransferases (DNMTs)

A

Catalyze DNA methylation using S-adenosyl methionine (SAMe) as donor

26
Q

Ten-Eleven Translocation (TET) Enzymes

A

Remove methylation

27
Q

Thymine-DNA Glycolase (TDG)

A

Removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond

28
Q

What modification is occuring in Histone Modification?

How does it occur?

What is the result?

What major enzymes cause this to occur?

A

Acetylation

Transfer of acetyl group from Acetyl Co-A to E-amino group of lysine

Partial neutralization of the (+) charge on histon, decreasing affinity to DNA

HAT (+ acetyl)

HDAC (- acetyl)

29
Q

HATs / HDACs

A

Histone Acetylransferases (HATs) - add acetyl to histone

Histone Deacetylase (HDACs) - remove acetyl from histone

30
Q

How are hypomethylating agents used?

What does it do?

A

Treatment of some medically fit patients with adverse features of AML

Blocks DNA Methyltransferae (Inhibits DNA methylation)

31
Q

HDAC Inhibitors?

A

Used in some individuals with lymphoid malignancies, such as multiple myeloma and Sezary Syndrome