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OS 201 Histo Exam 1 > Slide Prep > Flashcards

Slide Prep Flashcards

1
Q

The study of cells and their surrounding envi (extracellular components) and their organization into tissues and organs

A

Histology

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2
Q

The study of indiv cells

A

Cytology

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3
Q

Type of electron microscope that emits beams that pass over the tissue surface

A

Scanning EM

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4
Q

Type of electron microscope that emits beams that penetrate the tissue

Shows more detail

A

Transmission EM

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5
Q

Eyepiece magnification

A

10x

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6
Q

Scanner magnification

A

4x

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7
Q

LPO magnification

A

10x

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8
Q

HPO magnification

A

40x

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9
Q

OIO magnification

A

97-100x

Only used for peripheral blood and bone marrow

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10
Q

Angstrom value

A

0.1 nm / 10^-10 m

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11
Q

Diameter of HPO field of view

A

0.4 mm

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12
Q

Diameter of RBC

A

7.74 um

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13
Q

Steps of slide preparation

A
  1. Fixation
  2. Embedding
    a. Dehydration
    b. Clearing
  3. Staining
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14
Q

Step that preserves tissue morphology and architecture and prevents cell digestion

A

Fixation

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15
Q

2 examples of fixatives

A

Formaldehyde

Glutaraldehyde

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16
Q

Step for obtaining a rigid consistency for sectioning

A

Embedding

17
Q

Two steps that precede embedding

A

Dehydration

Clearing

18
Q

Reagent used for dehydration

A

EtOH (40, 60, 80, 90%)

19
Q

Lipid solvents that can be used for bathing in the clearing step

A

Benzene or xylene

Water and alcohol not miscible with paraffin

Xylene miscible with paraffin

20
Q

Machine used for sectioning

A

Microtome

21
Q

T/F

Slides for alight microscope can be used for an electron microscope

A

False

22
Q

Basic dyes react with?

Acidic dyes react with?

A

Acidic structures (basophilic)

Basic structures (acidophilic)

23
Q

Structures stained by hematoxylin

And also color

A

Blue

Nucleus (DNA, RNA)
Acidic regions of cytoplasm
Cartilage matrix
Ribosomes

(Acidic structures)

24
Q

Structures stained by eosin

And color

A

Pink

Basic regions of cytoplasm
Collagen fibers

25
Q

Structures stained by PAS

And color

A

Magenta

Glycogen
Carb rich molecules

26
Q

Structures stained by Wright and Giemsa stain

And color

A

Pink

  • erythrocytes
  • eosinophil granules

Purple

  • leukocyte nuclei
  • basophil granules

Blue
- cytoplasm of monocytes and lymphocytes

27
Q

Structures stained by Orcein Elastic Stain

And color

A

Brown

Elastic fibers

28
Q

Structures stained by silver stain

And color

A

Black

Reticular fibers

29
Q

Structures stained by iron hematoxylin

And color

A

Black

Muscle striations
Nuclei
Erythrocytes

30
Q

Structures stained by Massom’s Trichome

And color

A

Dark blue
- nuclei

Red

  • muscle
  • cytoplasm

Light blue
- collagen

OS 201 Histo Exam 1 (5 decks)

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