Skin Grafts

Question 1

What is the most common donor site for skin grafts?

Answer 1

Cranial lower lateral thoracic area

Question 2

What are the main caused of graft failure?

Answer 2

• Separation
• Movement
• Infection

Disrupt the fibrin bonds that bind the graft to the bed, impairing neovascularisation and nutrition of the graft

Question 3

How can infection cause seperation of the graft from the recipient bed?

Answer 3

Bacterial enzymes may cause dissolution of fibrin attachments
- Beta-haemolytic Strep and Pseudomonas produce large amounts of plasmin and proteolytic enzymes
- Pseudomonas also degrade elastin due to production of elastase (elastin facilitates adhesion through its adherence to fibrin)

Question 4

Which grafts lead to the best cosmetic outcome/best hair regrowth?

Answer 4

• Full thickness sheet grafts
• Unexpanded mesh grafts

Question 5

By what time must regeneration overtake degeneration for a graft to survive?

Answer 5

By day 7-8

Question 6

What are the 2 phases of graft adherence?

Answer 6

• Phase I - Attachment largely dependant on fibrin strands, forming links between collagen and elastin on each surface. Greatest gain over the initial 8 hours
• Phase II - Begins at approx 72hr. Fibrinous network is invaded by fibroblasts, leucocytes and phagocytes which begin the conversion into a fibrous adhesion. Continues to gain strength until a complete fibrous union is formed at day 10

Question 7

What is plasmatic imbibition

Answer 7

Nourishment of the graft until it revascularised via dilation of graft vessels, pulling fibrinogen-free, serum-like fluid and cells (erythrocytes and neutrophils) which have accumulated between the graft and recipient bed, into the vessels by capillary action
- Absorbed fluid diffuses into interstitial space cause peak oedema at 48-72hr

Question 8

Define inosculation

Answer 8

The anastomosis of the cut ends of graft vessels with recipient bed vessels of approx the same diameter
- Most commonly seen between 48-72hr

Question 9

What is vascular ingrowth?

Answer 9

Revascularisation of grafts by the ingrowth of new vessles from the bed into the graft
- Grow at approx 0.5mm/day
- Vessel maturation begins within 48hr

Question 10

What growth factor is elevated in the graft tissue which corresponds with peak vascular ingrowth activity?

Answer 10

VEGF

Question 11

What growth factor is elevated in the graft tissue which corresponds with peak vascular ingrowth activity?

Answer 11

VEGF

Question 12

Describe the expected changed in graft appearance

Answer 12

• Initially pale
• First 48hr, inosculation begins and associated oedema and vasc congestion - red to dark purple
• 72-96hr - lighter reddish hue
• 7-8 days - entire graft red-to-pink if survival is complete
• Day 14 - more normal, pale pink colour

Areas of avascular necrosis are persistently pale
Areas of ischaemic necrosis may appear black

Question 13

List the options for harvesting a split thickness skin graft

Answer 13

• Manual operated graft knife (Goulian-type graft knife, Humby and Watcon graft knife)
• Scapel blade
• Power-driver dermatome

Question 14

How long is splinting required after skin graft placement on a limb?

Answer 14

Until the fibrous tissue anchourage is strong enough to withstand shearing strain without capillary rupture (approx 10-14 days)

Question 15

List some advantages and disadvantages of split thickness grafts

Answer 15

Advantages
- Better viability than full thickness (89 vs 58% survival)
- Ingrowing vessels have less distance to travel
- Shorter diffusion distance
- Greater explansion

Disadvantages
- Less durable and more subject to trauma
- Absent or sparca hair regrowth
- Sacly appearance due to lack of senaceous glands
- Expensive equiment

Question 16

List some benefits of mesh grafts

Answer 16

• Drainage
• Flexibility
• Conformity
• Expansion

Question 17

What must be done before placement of any full thickness skin graft?

Answer 17

Removal of all subcutaneous tissue from graft

Question 18

How do you create a mesh graft?

Answer 18

• Use a #11 blade
• parallel rows of staggered incisions
• each incision 1-2cm long and spaced 0.5-2cm apart

ALternatively can use a mesh graft explansion unit

Question 19

What is the recommended explansion ratio for dogs and cats?

Answer 19

3:1 to 4:1

Question 20

What is the usual cause of infection of a skin graft?
How is it managed?

Answer 20

• Caused by overgrowth of normal skin organisms on abnormal skin
• Gentle cleaning of surface using 0.05% chlorhexidine with topical broad-spectrum ABx ointment

Question 21

What are the benefits of hyperbaric oxygen therapy in graft healing?

Answer 21

NONE! Contraindicated
- less granulation tissue production
- More inflammation
- Less percentage viability
- Only 13% graft viability at 10 days
- Reduced vascular ingrowth

Question 22

What support is there for using NPWT in the acute treatment of skin grafts?

Answer 22

• Earleir appearance of granulation tissue
• More rapid contraction of mesh holes
• Earlier adherence
• Reduced early graft necrosis at day 10 (1% vs 10%)

Question 23

List advantages and disadvantages of mesh grafts

Answer 23

Advantages
- Excellent viability (90-100% take)
- Improved drainage
- Improved conformability
- Additional stabilisation as granulation tissue grows into holes which also provded a vascular supply to lateral aspect of mesh holes

DIsadvantages:
- Excess granulation may grow through holes and cover top of graft

Question 24

Should full central sutures be placed in full thickness non-mesh grafts?
Why?

Answer 24

No - may cause haemorrhage and haematoma formation under a graft with limited drainage

Question 25

List the advantages and disadvantages of a full thickness un-meshed graft

Answer 25

Advantages
- Become pliable and movable
- Resist trauma
- More like normal skin
- Contraction is minimal
- Provide good protection
- No expensive equipment required

Disadvantages
- Do not survive as well as split-thickness grafts in the presence of infection
- Require drainage to survive as well as meshed grafts

Question 26

What sized biopsy punch is used for punch graft harvesting?

Answer 26

5mm - all SQ tissue must be removed

Question 27

How are pinch and punch grafts placed into the recipient bed?

Answer 27

• Pinch: Small slits made into granulation bed, approx 2-4mm deep and wide and 5-7mm apart, made at 2-=3- degree angle to surface. Each individual graft is tucked deep into a pocket
• Punch - 4mm biopsy punch used to make holes in granulation tissue approx 1-2cm apart in staggered rows. Cotton tipped applicator placed in holes for 5 mins for haemostasis prior to placement of individual punch grafts

Question 28

List advantages and diadvantages of pinch and punch grafts

Answer 28

Advantages:
- Simple
- No special equipment
- Take quickly and reliably
- Allow drainage
- Withstand infection well

Disadvantages
- Excessive bleeding may float graft out of recipient pocket or delay revascularisation
- Poor cosmetic appearance - sparce hair coat, dry and scaly
- Delicate and prone to injury

Question 29

What is a stamp graft?

Answer 29

“Chessboard graft”
Split or full thickness graft cut into squared 0.5-2cm and placed onto recipient bed with 1-10mm space between grafts

Question 30

What have mucosal grafts been described for?

Answer 30

• Replacement of nictitans membrane
• Extension of hypoplastic prepuce
• Conjunctival replacement
• Reconstruction of nasal passage
• Urethroplasty

Question 31

Where are mucosal grafts harvested from?

Answer 31

The buccal or sublingual mucosa (avoiding sublingual salivary duct and sublingual vessels)