Sir Gerard Ghani Lec

Question 1

Practices implemented to prevent accidental/unintentional release/exposure to pathogens

Answer 1

Biosafety

Question 2

Designed to prevent loss, theft, misuse, diversion or intentional release of pathogens

Answer 2

Biosecurity

Question 3

Protecting people from bad bugs

Answer 3

Biosafety

Question 4

Protecting bad bugs from bad people

Answer 4

Biosecurity

Question 5

unauthorized access in the laboratory

Answer 5

Biosecurity

Question 6

Recapping of needles

Answer 6

Biosafety

Question 7

Wearing of PPE

Answer 7

Biosafety

Question 8

Work overload

Answer 8

Neither

Question 9

Staff with huge debt

Answer 9

Neither

Question 10

Laboratory acquired infections

Answer 10

Biosafety

Question 11

Leakage of lab data

Answer 11

Biosecurity

Question 12

Lab technologist is a member of terrorist group

Answer 12

Biosecurity

Question 13

Lack of training

Answer 13

Neither

Question 14

Disgruntled staff

Answer 14

Biosecurity

Question 15

effective management of risks posed by working with infectious agents and toxins in laboratories. Its goal is to identify and reduce risk before they evolve into neear incidents

Answer 15

Biorisk management

Question 16

3 components of Biorisk management

Answer 16

Assessment, Mitigation, and Performance

Question 17

Enumerate the steps in Donning of PPE (in order)

Answer 17

Hand hygiene-Shoe cover-Hand hygiene-Inner gown-inner gloves-N95 mask-head cap-outer gown-face shield-outer gloves

Question 18

Enumerate the steps in Doffing of PPE (in order)

Answer 18

Outer gloves-outer gown-shoe cover-face shield-head cap-inner gloves-inner gown-hand hygiene-N95 mask-hand hygiene

Question 19

This equipment is used for preparing Mastermix

Answer 19

PCR Cabinet (Reagent preparation room)

Question 20

It is used for RNA Extraction and Template Addition

Answer 20

Biosafety cabinet class II (Extraction room)

Question 21

used for the treatment of biohazardous wastes

Answer 21

Autoclave (Doffing area)

Question 22

What is the principle of autoclave?

Answer 22

Steam under pressure

Question 23

Thermal cycler is also termed as _______room

Answer 23

Amplification room

Question 24

2 types of waste generated by molecular laboratory

Answer 24

General waste and Biohazardous waste

Question 25

TRUE OR FALSE | DNA and RNA are macromolecules comprised of many nucleotides

Answer 25

TRUE

Question 26

3 parts of a nucleotide

Answer 26

A nitrogenous base, 5 carbon sugar, and 1 Phosphate group

Question 27

What enzymes unwind the DNA?

Answer 27

DNA helicase

Question 28

This enzyme is for the synthesis of short RNA primers

Answer 28

RNA primase

Question 29

adds nucleotides to the primers and extends the strand

Answer 29

DNA polymerase III

Question 30

degrades the RNA primer and replaces it with DNA

Answer 30

DNA Polymerase I

Question 31

connect two strands of RNA

Answer 31

DNA ligase

Question 32

Synthesis of RNA (mRNA) through DNA by RNA polymerase

Answer 32

Transcription

Question 33

Synthesis of polypeptide/protein through mRNA

Answer 33

Translation

Question 34

Where does translation happen?

Answer 34

in the ribosomes