Lab Techniques in Molecular Diagnostics Flashcards
1
Q
Mitochondrial DNA usually obtained from what spx?
A
Hair follicles
2
Q
True or False:
Bacteria must be treated with enzymes and detergents that destroy their cell walls in order to release nucleic acids
A
True
3
Q
How to inactivate RNAse
A
heat sample at 60C for 10 minutes
4
Q
RNAse inhibitor
A
Guanidine thiocyanate
5
Q
Reducing agent
A
2-beta-mercaptoethanol
6
Q
Give the five Isolation methods
A
Organic Inorganic Solid phase Chelex extraction Isolation of poly-A-enriched RNA (mRNA)
